NUP2/YLR335W Summary Help

Standard Name NUP2 1
Systematic Name YLR335W
Feature Type ORF, Verified
Description Nucleoporin involved in nucleocytoplasmic transport; binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description NUclear Pore 1
Chromosomal Location
ChrXII:797430 to 799592 | ORF Map | GBrowse
Gene Ontology Annotations All NUP2 GO evidence and references
  View Computational GO annotations for NUP2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Classical genetics
Large-scale survey
190 total interaction(s) for 88 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 61
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 19
  • Biochemical Activity: 1
  • Co-fractionation: 1
  • Co-purification: 3
  • FRET: 2
  • PCA: 5
  • Reconstituted Complex: 9
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 49
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 1
  • Positive Genetic: 7
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 12

Expression Summary
Length (a.a.) 720
Molecular Weight (Da) 77,880
Isoelectric Point (pI) 7.34
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:797430 to 799592 | ORF Map | GBrowse
Last Update Coordinates: 2004-02-05 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2163 797430..799592 2004-02-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004327

NUP2 encodes a non-essential nuclear pore protein that has a central domain similar to those of Nsp1p and Nup1p (1, 6, 7). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 6, 7, 8, 9). The structure of the vertebrate NPC has been studied extensively; recent reviews include 10, 11, 12, and 13. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (14, 15). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 6, 7, 16, 17, 18). nup2 mutants show genetic interactions with nsp1 and nup1 conditional alleles (1, 6, 7). Nup1p interacts with the nuclear import factor Srp1p (19) and with the small GTPase Ran (encoded by GSP1) (20).

Last updated: 1999-07-18 Contact SGD

References cited on this page View Complete Literature Guide for NUP2
1) Loeb JD, et al.  (1993) NUP2, a novel yeast nucleoporin, has functional overlap with other proteins of the nuclear pore complex. Mol Biol Cell 4(2):209-22
2) Hood JK, et al.  (2000) Nup2p is located on the nuclear side of the nuclear pore complex and coordinates Srp1p/importin-alpha export. J Cell Sci 113 ( Pt 8):1471-80
3) Solsbacher J, et al.  (2000) Nup2p, a yeast nucleoporin, functions in bidirectional transport of importin alpha. Mol Cell Biol 20(22):8468-79
4) Ishii K, et al.  (2002) Chromatin boundaries in budding yeast: the nuclear pore connection. Cell 109(5):551-62
5) Dilworth DJ, et al.  (2001) Nup2p dynamically associates with the distal regions of the yeast nuclear pore complex. J Cell Biol 153(7):1465-78
6) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
7) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
8) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
9) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
10) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
11) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
12) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
13) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
14) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
15) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
16) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
17) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
18) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
19) Belanger KD, et al.  (1994) Genetic and physical interactions between Srp1p and nuclear pore complex proteins Nup1p and Nup2p. J Cell Biol 126(3):619-30
20) Dingwall C, et al.  (1995) A family of Ran binding proteins that includes nucleoporins. Proc Natl Acad Sci U S A 92(16):7525-9