NUP159/YIL115C Summary Help

NUP159 BASIC INFORMATION

Standard Name NUP159
Systematic Name YIL115C
Alias NUP158 , RAT7
Feature Type ORF, Verified
Description Nucleoporin, subunit of the nuclear pore complex that is found exclusively on the cytoplasmic side, member of the Nup82 subcomplex, required for mRNA export (1, 2, 3, 4 and see Summary Paragraph)
Name Description NUclear Pore
GO Annotations All NUP159 GO evidence and references
    View Computational GO annotations for NUP159
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All NUP159 Phenotype details and references
Classical genetics
conditional
reduction of function
unspecified
Large-scale survey
null
Interactions NUP159 All interactions details and references
119 total interaction(s) for 65 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 36
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 18
  • Co-purification: 2
  • PCA: 3
  • Reconstituted Complex: 8
  • Two-hybrid: 4
Genetic Interactions
  • Dosage Rescue: 5
  • Phenotypic Enhancement: 13
  • Phenotypic Suppression: 17
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 8
  • Synthetic Rescue: 3
Sequence Information
ChrIX:148706 to 144324 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 1994-12-10 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..4383 148706..144324 1994-12-10 1994-12-10
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000001377

NUP159 RESOURCES

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Expression Summary histogram

ADDITIONAL INFORMATION for NUP159

SUMMARY PARAGRAPH for NUP159

NUP159 encodes an essential nuclear pore protein (5, 6, 7). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 7, 8, 9, 10). The structure of the vertebrate NPC has been studied extensively; recent reviews include 11, 12, 13, and 14. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (15, 16). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 7, 8, 17, 18, 19). Nup159p is one of a group of nucleoporins that contain multiple repeats of the amino acids GLFG (5, 6, 7), and is found only on the cytoplasmic face of the pore (6). At the restrictive temperature, a temperature-sensitive nup159 allele changes the distribution of NPCs within the nuclear envelope, and is defective in mRNA export from the nucleus (5). The phenotype can be partially suppressed by overexpression of the RNA export factor Gle1p (20, 7). Domain analysis suggests that the N-terminal thrid of Nup159p is important for its role in nuclear mRNA export (21). While nup159 mutants do not show protein import defects (7), Nup159p associates physically with Pse1p, a member of a protein family implicated in nuclear protein import (22). nup159 mutations are synthetically lethal with mutations in several other nucleoporin genes (7).

Last updated: 1999-08-12

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for NUP159]

1) Hodge CA, et al.  (1999) Rat8p/Dbp5p is a shuttling transport factor that interacts with Rat7p/Nup159p and Gle1p and suppresses the mRNA export defect of xpo1-1 cells. EMBO J 18(20):5778-88
2) Rout MP, et al.  (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 148(4):635-51
3) Bailer SM, et al.  (2000) Nup116p associates with the Nup82p-Nsp1p-Nup159p nucleoporin complex. J Biol Chem 275(31):23540-8
4) Lutzmann M, et al.  (2005) Reconstitution of Nup157 and Nup145N into the Nup84 complex. J Biol Chem 280(18):18442-51
5) Gorsch LC, et al.  (1995) A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes. J Cell Biol 129(4):939-55
6) Kraemer DM, et al.  (1995) The essential yeast nucleoporin NUP159 is located on the cytoplasmic side of the nuclear pore complex and serves in karyopherin-mediated binding of transport substrate. J Biol Chem 270(32):19017-21
7) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
8) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
9) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
10) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
11) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
12) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
13) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
14) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
15) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
16) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
17) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
18) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
19) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
20) Del Priore V, et al.  (1996) The product of the Saccharomyces cerevisiae RSS1 gene, identified as a high-copy suppressor of the rat7-1 temperature-sensitive allele of the RAT7/NUP159 nucleoporin, is required for efficient mRNA export. Mol Biol Cell 7(10):1601-21
21) Del Priore V, et al.  (1997) A structure/function analysis of Rat7p/Nup159p, an essential nucleoporin of Saccharomyces cerevisiae. J Cell Sci 110 ( Pt 23):2987-99
22) Seedorf M, et al.  (1999) Interactions between a nuclear transporter and a subset of nuclear pore complex proteins depend on Ran GTPase. Mol Cell Biol 19(2):1547-57