NUP145/YGL092W Summary Help

Standard Name NUP145 1, 2
Systematic Name YGL092W
Alias RAT10
Feature Type ORF, Verified
Description Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 (3, 4, 5, 6, 7, 8, 9, 10 and see Summary Paragraph)
Name Description NUclear Pore 11
Chromosomal Location
ChrVII:337906 to 341859 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All NUP145 GO evidence and references
  View Computational GO annotations for NUP145
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Classical genetics
reduction of function
repressible
Large-scale survey
null
reduction of function
repressible
Resources
151 total interaction(s) for 44 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 74
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 17
  • Co-crystal Structure: 5
  • Co-purification: 3
  • FRET: 6
  • PCA: 8
  • Reconstituted Complex: 17
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Lethality: 1
  • Phenotypic Enhancement: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 9

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,317
Molecular Weight (Da) 145,660
Isoelectric Point (pI) 5.51
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:337906 to 341859 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3954 337906..341859 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003060
SUMMARY PARAGRAPH for NUP145

NUP145 encodes an essential nuclear pore protein (12). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 12, 13, 14, 15). The structure of the vertebrate NPC has been studied extensively; recent reviews include 16, 17, 18, and 19. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (20, 21). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 12, 13, 22, 23, 24). The N- and C-terminal domains of Nup145p are functionally distinct (25, 26, 4) and are cleaved in vivo (26, 4), though the cleavage is not essential for Nup145p function (26). The N-terminal half of Nup145p contains multiple repeats of the amino acids GLFG (27, 12) and is similar to Nup100p and Nup116p (1, 12). The C-terminal half assembles into the NPC subcomplex containing Nup84p and several other nucleoporins (4, 28, 12). Mutations in NUP145 cause defects in nuclear protein import and nuclear RNA export (2, 29, 12, 26, 25). nup145 mutations are also synthetically lethal with mutations in several other nucleoporin genes (12). A related nucleoporin, Nup98, has been identified in vertebrates (12).

Last updated: 1999-08-10 Contact SGD

References cited on this page View Complete Literature Guide for NUP145
1) Wente SR and Blobel G  (1994) NUP145 encodes a novel yeast glycine-leucine-phenylalanine-glycine (GLFG) nucleoporin required for nuclear envelope structure. J Cell Biol 125(5):955-69
2) Fabre E, et al.  (1994) Nup145p is required for nuclear export of mRNA and binds homopolymeric RNA in vitro via a novel conserved motif. Cell 78(2):275-89
3) Teixeira MT, et al.  (1999) Self-catalyzed cleavage of the yeast nucleoporin Nup145p precursor. J Biol Chem 274(45):32439-44
4) Teixeira MT, et al.  (1997) Two functionally distinct domains generated by in vivo cleavage of Nup145p: a novel biogenesis pathway for nucleoporins. EMBO J 16(16):5086-97
5) Menon BB, et al.  (2005) Reverse recruitment: the Nup84 nuclear pore subcomplex mediates Rap1/Gcr1/Gcr2 transcriptional activation. Proc Natl Acad Sci U S A 102(16):5749-54
6) Therizols P, et al.  (2006) Telomere tethering at the nuclear periphery is essential for efficient DNA double strand break repair in subtelomeric region. J Cell Biol 172(2):189-99
7) Fernandez-Martinez J and Rout MP  (2009) Nuclear pore complex biogenesis. Curr Opin Cell Biol 21(4):603-12
8) Fiserova J, et al.  (2010) Facilitated transport and diffusion take distinct spatial routes through the nuclear pore complex. J Cell Sci 123(Pt 16):2773-80
9) Tous C, et al.  (2011) A novel assay identifies transcript elongation roles for the Nup84 complex and RNA processing factors. EMBO J 30(10):1953-64
10) Fernandez-Martinez J, et al.  (2012) Structure-function mapping of a heptameric module in the nuclear pore complex. J Cell Biol 196(4):419-34
11) Davis LI and Fink GR  (1990) The NUP1 gene encodes an essential component of the yeast nuclear pore complex. Cell 61(6):965-78
12) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
13) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
14) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
15) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
16) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
17) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
18) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
19) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
20) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
21) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
22) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
23) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
24) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
25) Dockendorff TC, et al.  (1997) C-terminal truncations of the yeast nucleoporin Nup145p produce a rapid temperature-conditional mRNA export defect and alterations to nuclear structure. Mol Cell Biol 17(2):906-20
26) Emtage JL, et al.  (1997) Defining the essential functional regions of the nucleoporin Nup145p. J Cell Sci 110 ( Pt 7):911-25
27) Wente SR, et al.  (1992) A new family of yeast nuclear pore complex proteins. J Cell Biol 119(4):705-23
28) Siniossoglou S, et al.  (1996) A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores. Cell 84(2):265-75
29) Sharma K, et al.  (1996) Yeast nucleoporin mutants are defective in pre-tRNA splicing. Mol Cell Biol 16(1):294-301