NUP116/YMR047C Summary Help

Standard Name NUP116 1
Systematic Name YMR047C
Alias NSP116
Feature Type ORF, Verified
Description FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p); NUP116 has a paralog, NUP100, that arose from the whole genome duplication (2, 3, 4, 5, 6, 7, 8, 9, 10, 11 and see Summary Paragraph)
Name Description NUclear Pore 12
Chromosomal Location
ChrXIII:366705 to 363364 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NUP116 GO evidence and references
  View Computational GO annotations for NUP116
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
conditional
null
overexpression
unspecified
Large-scale survey
null
reduction of function
Resources
275 total interaction(s) for 136 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 78
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 26
  • Co-fractionation: 2
  • Co-purification: 63
  • FRET: 5
  • PCA: 4
  • Protein-peptide: 1
  • Reconstituted Complex: 16
  • Two-hybrid: 29

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 5
  • Negative Genetic: 15
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 4
  • Positive Genetic: 1
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 14
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,113
Molecular Weight (Da) 116,234
Isoelectric Point (pI) 10.13
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:366705 to 363364 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3342 366705..363364 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004650
SUMMARY PARAGRAPH for NUP116

NUP116 encodes a nuclear pore protein (1, 13). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 13, 14, 15, 16). The structure of the vertebrate NPC has been studied extensively; recent reviews include 17, 18, 19, and 20. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (21, 22). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 13, 14, 23, 24, 25). NUP116 is not essential, but the null mutant is temperature sensitive, and nup116 mutants have defects in nuclear RNA export and nuclear tRNA export and altered nuclear envelope morphology (26, 2, 13, 27). Nup116p is one of a group of nucleoporins that contain multiple repeats of the amino acids GLFG (1, 28, 13). Nup100p and Nup116p are similar over their entire lengths, and may have arisen by gene duplication (1, 29, 13). Deletion of the GLFG repeats from Nup116p causes a defect in nuclear protein import (4, 13). Nup116p interacts with two nuclear transport factors, Kap95p (4) and Pse1p (30). The interaction with Kap95p involves the GLFG repeats of Nup116p (4). Nup116p is stoichiometrically bound to the mRNA transport factor Gle2p, forming a stable complex, via a short sequence (the GLEBS) also found in nucleoporins from rat and Xenopus (31). A related nucleoporin, Nup98, has been identified in vertebrates (13, 32, 33).

Last updated: 1999-08-06 Contact SGD

References cited on this page View Complete Literature Guide for NUP116
1) Wente SR, et al.  (1992) A new family of yeast nuclear pore complex proteins. J Cell Biol 119(4):705-23
2) Sharma K, et al.  (1996) Yeast nucleoporin mutants are defective in pre-tRNA splicing. Mol Cell Biol 16(1):294-301
3) Rout MP, et al.  (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 148(4):635-51
4) Iovine MK, et al.  (1995) The GLFG repetitive region of the nucleoporin Nup116p interacts with Kap95p, an essential yeast nuclear import factor. J Cell Biol 131(6 Pt 2):1699-713
5) Stage-Zimmermann T, et al.  (2000) Factors affecting nuclear export of the 60S ribosomal subunit in vivo. Mol Biol Cell 11(11):3777-89
6) Strawn LA, et al.  (2001) The GLFG regions of Nup116p and Nup100p serve as binding sites for both Kap95p and Mex67p at the nuclear pore complex. J Biol Chem 276(9):6445-52
7) Pyhtila B and Rexach M  (2003) A gradient of affinity for the karyopherin Kap95p along the yeast nuclear pore complex. J Biol Chem 278(43):42699-709
8) Lutzmann M, et al.  (2005) Reconstitution of Nup157 and Nup145N into the Nup84 complex. J Biol Chem 280(18):18442-51
9) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
10) Patel SS, et al.  (2007) Natively unfolded nucleoporins gate protein diffusion across the nuclear pore complex. Cell 129(1):83-96
11) Terry LJ and Wente SR  (2007) Nuclear mRNA export requires specific FG nucleoporins for translocation through the nuclear pore complex. J Cell Biol 178(7):1121-32
12) Davis LI and Fink GR  (1990) The NUP1 gene encodes an essential component of the yeast nuclear pore complex. Cell 61(6):965-78
13) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
14) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
15) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
16) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
17) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
18) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
19) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
20) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
21) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
22) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
23) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
24) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
25) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
26) Wente SR and Blobel G  (1993) A temperature-sensitive NUP116 null mutant forms a nuclear envelope seal over the yeast nuclear pore complex thereby blocking nucleocytoplasmic traffic. J Cell Biol 123(2):275-84
27) Sarkar S and Hopper AK  (1998) tRNA nuclear export in saccharomyces cerevisiae: in situ hybridization analysis. Mol Biol Cell 9(11):3041-55
28) Wimmer C, et al.  (1992) A new subclass of nucleoporins that functionally interact with nuclear pore protein NSP1. EMBO J 11(13):5051-61
29) Wente SR and Blobel G  (1994) NUP145 encodes a novel yeast glycine-leucine-phenylalanine-glycine (GLFG) nucleoporin required for nuclear envelope structure. J Cell Biol 125(5):955-69
30) Seedorf M, et al.  (1999) Interactions between a nuclear transporter and a subset of nuclear pore complex proteins depend on Ran GTPase. Mol Cell Biol 19(2):1547-57
31) Bailer SM, et al.  (1998) Nup116p and nup100p are interchangeable through a conserved motif which constitutes a docking site for the mRNA transport factor gle2p. EMBO J 17(4):1107-19
32) Pritchard CE, et al.  (1999) RAE1 is a shuttling mRNA export factor that binds to a GLEBS-like NUP98 motif at the nuclear pore complex through multiple domains. J Cell Biol 145(2):237-54
33) Fontoura BM, et al.  (1999) A conserved biogenesis pathway for nucleoporins: proteolytic processing of a 186-kilodalton precursor generates Nup98 and the novel nucleoporin, Nup96. J Cell Biol 144(6):1097-112