NUP1/YOR098C Summary Help

Standard Name NUP1 1
Systematic Name YOR098C
Feature Type ORF, Verified
Description FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of thenuclear pore complex (NPC) permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153 (1, 2, 3, 4, 5, 6, 7, 8 and see Summary Paragraph)
Name Description NUclear Pore 1
Chromosomal Location
ChrXV:511178 to 507948 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NUP1 GO evidence and references
  View Computational GO annotations for NUP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
reduction of function
repressible
Resources
130 total interaction(s) for 68 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 45
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 18
  • Biochemical Activity: 1
  • Co-crystal Structure: 2
  • Co-fractionation: 1
  • Co-purification: 1
  • FRET: 4
  • PCA: 2
  • Protein-RNA: 1
  • Reconstituted Complex: 16
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 19
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,076
Molecular Weight (Da) 113,581
Isoelectric Point (pI) 10.42
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:511178 to 507948 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3231 511178..507948 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005624
SUMMARY PARAGRAPH for NUP1

NUP1 encodes a nuclear pore protein (nucleoporin) that was first identified by cross-reaction with antibodies raised against rat liver nucleoporins (1). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 9, 10, 11, 12). The structure of the vertebrate NPC has been studied extensively; recent reviews include 13, 14, 15, and 16. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (17, 18). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 9, 10, 19, 20, 21). Nup1p is one of a group of nucleoporins that contain multiple repeats of the amino acids FXFG (1, 10, 9). Mutations in NUP1 cause defects in both protein import into and mRNA export out of the nucleus, as well as invaginations of the nuclear envelope (2, 9, 10). Davis and Fink (1) report that a NUP1 deletion is inviable, whereas others (22, 23) report that a NUP1 deletion is viable. The difference is attributed the difference to a naturally occurring suppressor present in some strains (22). Several other nucleoporin genes have been identified in yeast by screening for mutations that are synthetically lethal with conditional nup1 mutations (24, 9, 10, 19). NUP1 shows genetic interactions with SRP1, which encodes a nuclear import factor (22, 9, 10), and with ARP2 (25).

Last updated: 1999-07-26 Contact SGD

References cited on this page View Complete Literature Guide for NUP1
1) Davis LI and Fink GR  (1990) The NUP1 gene encodes an essential component of the yeast nuclear pore complex. Cell 61(6):965-78
2) Bogerd AM, et al.  (1994) nup1 mutants exhibit pleiotropic defects in nuclear pore complex function. J Cell Biol 127(2):319-32
3) Titov AA and Blobel G  (1999) The karyopherin Kap122p/Pdr6p imports both subunits of the transcription factor IIA into the nucleus. J Cell Biol 147(2):235-46
4) Stage-Zimmermann T, et al.  (2000) Factors affecting nuclear export of the 60S ribosomal subunit in vivo. Mol Biol Cell 11(11):3777-89
5) Gilchrist D, et al.  (2002) Accelerating the rate of disassembly of karyopherin.cargo complexes. J Biol Chem 277(20):18161-72
6) Pyhtila B and Rexach M  (2003) A gradient of affinity for the karyopherin Kap95p along the yeast nuclear pore complex. J Biol Chem 278(43):42699-709
7) Strawn LA, et al.  (2004) Minimal nuclear pore complexes define FG repeat domains essential for transport. Nat Cell Biol 6(3):197-206
8) Patel SS, et al.  (2007) Natively unfolded nucleoporins gate protein diffusion across the nuclear pore complex. Cell 129(1):83-96
9) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
10) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
11) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
12) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
13) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
14) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
15) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
16) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
17) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
18) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
19) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
20) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
21) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
22) Belanger KD, et al.  (1994) Genetic and physical interactions between Srp1p and nuclear pore complex proteins Nup1p and Nup2p. J Cell Biol 126(3):619-30
23) Schlaich NL and Hurt EC  (1995) Analysis of nucleocytoplasmic transport and nuclear envelope structure in yeast disrupted for the gene encoding the nuclear pore protein Nup1p. Eur J Cell Biol 67(1):8-14
24) Kenna MA, et al.  (1996) Yeast N1e3p/Nup170p is required for normal stoichiometry of FG nucleoporins within the nuclear pore complex. Mol Cell Biol 16(5):2025-36
25) Yan C, et al.  (1997) A role for the divergent actin gene, ACT2, in nuclear pore structure and function. EMBO J 16(12):3572-86