NUM1/YDR150W Summary Help

Standard Name NUM1 1
Systematic Name YDR150W
Alias PAC12 2
Feature Type ORF, Verified
Description Protein required for nuclear migration; component of the mitochondria-ER-cortex-ancor (MECA); required for the association of mitochondria with the cell cortex and for accurate distribution of mitochondrial network; interacts with Mdm36p to link the ER and motochondria at the cortex; localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex (1, 3, 4, 5)
Name Description NUclear Migration 1
Chromosomal Location
ChrIV:755628 to 763874 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All NUM1 GO evidence and references
  View Computational GO annotations for NUM1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 6 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
378 total interaction(s) for 215 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 17
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 5
  • Co-localization: 1
  • Co-purification: 1
  • FRET: 1
  • PCA: 3
  • Protein-RNA: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 51

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 175
  • Phenotypic Enhancement: 4
  • Positive Genetic: 27
  • Synthetic Growth Defect: 36
  • Synthetic Lethality: 48
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 2,748
Molecular Weight (Da) 313,030
Isoelectric Point (pI) 5.18
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:755628 to 763874 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..8247 755628..763874 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002557
References cited on this page View Complete Literature Guide for NUM1
1) Kormanec J, et al.  (1991) Nuclear migration in Saccharomyces cerevisiae is controlled by the highly repetitive 313 kDa NUM1 protein. Mol Gen Genet 230(1-2):277-87
2) Geiser JR, et al.  (1997) Saccharomyces cerevisiae genes required in the absence of the CIN8-encoded spindle motor act in functionally diverse mitotic pathways. Mol Biol Cell 8(6):1035-50
3) Farkasovsky M and Kuntzel H  (1995) Yeast Num1p associates with the mother cell cortex during S/G2 phase and affects microtubular functions. J Cell Biol 131(4):1003-14
4) Farkasovsky M and Kuntzel H  (2001) Cortical Num1p interacts with the dynein intermediate chain Pac11p and cytoplasmic microtubules in budding yeast. J Cell Biol 152(2):251-62
5) Lackner LL, et al.  (2013) Endoplasmic reticulum-associated mitochondria-cortex tether functions in the distribution and inheritance of mitochondria. Proc Natl Acad Sci U S A 110(6):E458-67