NUF2/YOL069W Summary Help

Standard Name NUF2 1
Systematic Name YOL069W
Feature Type ORF, Verified
Description Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p (1, 2, 3, 4)
Name Description NUclear Filament-containing protein 1
Chromosomal Location
ChrXV:198942 to 200297 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All NUF2 GO evidence and references
  View Computational GO annotations for NUF2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
conditional
repressible
Large-scale survey
conditional
null
reduction of function
repressible
unspecified
Resources
226 total interaction(s) for 123 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 22
  • Affinity Capture-Western: 19
  • Co-fractionation: 1
  • Co-localization: 2
  • Co-purification: 3
  • PCA: 18
  • Reconstituted Complex: 3
  • Two-hybrid: 147

Genetic Interactions
  • Dosage Rescue: 2
  • Synthetic Lethality: 9

Resources
Expression Summary
histogram
Resources
Length (a.a.) 451
Molecular Weight (Da) 52,973
Isoelectric Point (pI) 4.35
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:198942 to 200297 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1356 198942..200297 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005430
References cited on this page View Complete Literature Guide for NUF2
1) Osborne MA, et al.  (1994) Nuf2, a spindle pole body-associated protein required for nuclear division in yeast. J Cell Biol 125(4):853-66
2) Wigge PA and Kilmartin JV  (2001) The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J Cell Biol 152(2):349-60
3) Janke C, et al.  (2001) The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control. EMBO J 20(4):777-91
4) He X, et al.  (2001) Molecular analysis of kinetochore-microtubule attachment in budding yeast. Cell 106(2):195-206