NTH2/YBR001C Summary

Standard Name	NTH2
Systematic Name	YBR001C
Feature Type	ORF, Verified
Description	Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication (1, 2 and see Summary Paragraph)
Name Description	Neutral TreHalase

Chromosomal Location	ChrII:241283 to 238941 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All NTH2 GO evidence and references

	View Computational GO annotations for NTH2
Molecular Function
Manually curated	alpha,alpha-trehalase activity (IMP)
Biological Process
Manually curated	trehalose catabolic process (IMP)
Cellular Component
High-throughput	cytoplasm (IDA) mitochondrion (IDA)

Mutant phenotypes All NTH2 Phenotype evidence and references

Classical genetics
null	thermotolerance: decreased viable
Large-scale survey
null	competitive fitness: decreased starvation resistance: decreased viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All NTH2 Interaction evidence and references

	42 total interaction(s) for 32 unique genes/features.
Physical Interactions	Affinity Capture-MS: 8 Biochemical Activity: 1
Genetic Interactions	Dosage Rescue: 2 Negative Genetic: 22 Phenotypic Enhancement: 4 Positive Genetic: 3 Synthetic Growth Defect: 1 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All NTH2 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrII:241283 to 238941 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1997-01-28

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2343	241283..238941	2011-02-03	1997-01-28

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000205

SUMMARY PARAGRAPH for NTH2

NTH2 is 77% identical to NTH1, which encodes a cytoplasmic neutral trehalase enzyme. Although NTH2 mRNA has been identified by Northern analysis (1), it does not appear to encode significant trehalase activity, or be involved in trehalose catabolism, as an nth2 null mutant exhibits normal levels of both neutral trehalase activity and trehalose (1). An nth2 null mutant does display sensitivity to heat shock, indicating it may be involved in thermotolerance. NTH2 appears to be induced during stationary phase and repressed during exponential growth (1).

Last updated: 2005-08-12

References cited on this page View Complete Literature Guide for NTH2

1)	Nwaka S, et al. (1995) Expression and function of the trehalase genes NTH1 and YBR0106 in Saccharomyces cerevisiae. J Biol Chem 270(17):10193-8
2)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61