NTH1/YDR001C Summary

Standard Name	NTH1
Systematic Name	YDR001C
Feature Type	ORF, Verified
Description	Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description	Neutral TreHalase 3

Chromosomal Location	ChrIV:452475 to 450220 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All NTH1 GO evidence and references

	View Computational GO annotations for NTH1
Molecular Function
Manually curated	alpha,alpha-trehalase activity (TAS)
Biological Process
Manually curated	trehalose catabolic process (IMP)
Cellular Component
High-throughput	cytoplasm (IDA)

Pathways	trehalose catabolism

Mutant phenotypes All NTH1 Phenotype evidence and references

Classical genetics
null	trehalose accumulation: increased toxin resistance: increased viability: increased
overexpression	nuclear morphology: abnormal vegetative growth: arrested
Large-scale survey
null	competitive fitness: increased haploproficient resistance to cycloheximide: decreased resistance to amitrole: decreased resistance to sodium arsenite: decreased resistance to arsenite(3-): decreased vacuolar morphology: abnormal viable
overexpression	cell cycle progression: abnormal
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All NTH1 Interaction evidence and references

	95 total interaction(s) for 57 unique genes/features.
Physical Interactions	Affinity Capture-MS: 22 Affinity Capture-RNA: 1 Affinity Capture-Western: 6 Biochemical Activity: 9 Reconstituted Complex: 4 Two-hybrid: 4
Genetic Interactions	Dosage Rescue: 2 Negative Genetic: 27 Phenotypic Enhancement: 9 Positive Genetic: 8 Synthetic Growth Defect: 1 Synthetic Lethality: 1 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All NTH1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:452475 to 450220 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2256	452475..450220	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000002408

SUMMARY PARAGRAPH for NTH1

Trehalose is a storage carbohydrate that can either be synthesized by the cell or obtained from the external environment, and is converted by trehalase with water into two glucose molecules (6). S. cerevisiae has two trehalase enzymes, an acid trehalase encoded by ATH1 (7) and a neutral trehalase encoded by NTH1 (3). A third locus, NTH2, is 77% identical to NTH1, but does not appear to encode a trehalase activity, or be involved in trehalose catabolism, since an nth2 null mutant exhibits normal levels of neutral trehalase activity and trehalose (2).

NTH1 is induced by various stresses including exposure to heat, hydrogen peroxide, or cycloheximide (1). Nth1p is a cytoplasmic homodimer required for the hydrolysis of intracellular trehalose (3), and is involved in thermotolerance through a mechanism that does not appear to correspond to trehalose abundance (2). Phosphorylation by Cdc28p has been predicted to activate Nth1p. Deletion of NTH1 results in complete loss of neutral trehalase activity, accumulation of internal trehalose, and heat sensitivity.

Last updated: 2005-08-12

References cited on this page View Complete Literature Guide for NTH1

1)	Zahringer H, et al. (1997) Neutral trehalase Nth1p of Saccharomyces cerevisiae encoded by the NTH1 gene is a multiple stress responsive protein. FEBS Lett 412(3):615-20
2)	Nwaka S, et al. (1995) Expression and function of the trehalase genes NTH1 and YBR0106 in Saccharomyces cerevisiae. J Biol Chem 270(17):10193-8
3)	Kopp M, et al. (1993) Molecular analysis of the neutral trehalase gene from Saccharomyces cerevisiae. J Biol Chem 268(7):4766-74
4)	Ubersax JA, et al. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6)	Francois J and Parrou JL (2001) Reserve carbohydrates metabolism in the yeast Saccharomyces cerevisiae. FEMS Microbiol Rev 25(1):125-45
7)	Alizadeh P and Klionsky DJ (1996) Purification and biochemical characterization of the ATH1 gene product, vacuolar acid trehalase, from Saccharomyces cerevisiae. FEBS Lett 391(3):273-8