NTG1/YAL015C Summary

Standard Name	NTG1 1 (see Nomenclature conflict Note)
Systematic Name	YAL015C
Alias	FUN33 , SCR1 , ogg2 2 , 3
Feature Type	ORF, Verified
Description	DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication (4, 5, 6, 7, 8)
Name Description	eNdonuclease Three-like Glycosylase 1

Chromosomal Location	ChrI:128102 to 126903 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All NTG1 GO evidence and references

	View Computational GO annotations for NTG1
Molecular Function
Manually curated	DNA-(apurinic or apyrimidinic site) lyase activity (IDA) oxidized purine nucleobase lesion DNA N-glycosylase activity (IDA) oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity (IDA, ISS)
Biological Process
Manually curated	base-excision repair (IDA) base-excision repair, AP site formation (IDA) cellular response to oxidative stress (IMP) DNA repair (IDA) positive regulation of mitochondrial DNA replication (IMP)
Cellular Component
Manually curated	mitochondrion (IDA) nucleus (IDA)
High-throughput	mitochondrion (IDA)

Mutant phenotypes All NTG1 Phenotype evidence and references

Classical genetics
null	UV resistance: normal gamma ray resistance: normal mitochondrial genome maintenance: abnormal mutation frequency: decreased mutation frequency: increased resistance to hydrogen peroxide: decreased resistance to menadione: decreased viable
Large-scale survey
null	UV resistance: decreased glycogen accumulation: decreased competitive fitness: increased resistance to 2-phenyl-3-nitroso-imidazo[1,2-a]pyrimidine: decreased viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All NTG1 Interaction evidence and references

	70 total interaction(s) for 39 unique genes/features.
Physical Interactions	Affinity Capture-MS: 14 Affinity Capture-RNA: 3 Affinity Capture-Western: 1 Co-purification: 1
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 1 Phenotypic Enhancement: 19 Phenotypic Suppression: 6 Positive Genetic: 2 Synthetic Growth Defect: 14 Synthetic Lethality: 7 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All NTG1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrI:128102 to 126903 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1200	128102..126903	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000013

NOMENCLATURE CONFLICT NOTE

Name	Relevance	Description
SCR1	Nomenclature conflict	Both NTG1/YAL015C, a glycosylase involved in DNA repair, and SCR1, a small cytoplasmic RNA, have been referred to as SCR1.

References cited on this page View Complete Literature Guide for NTG1

1)	Eide L, et al. (1996) Base excision of oxidative purine and pyrimidine DNA damage in Saccharomyces cerevisiae by a DNA glycosylase with sequence similarity to endonuclease III from Escherichia coli. Proc Natl Acad Sci U S A 93(20):10735-40
2)	Nash HM, et al. (1996) Cloning of a yeast 8-oxoguanine DNA glycosylase reveals the existence of a base-excision DNA-repair protein superfamily. Curr Biol 6(8):968-80
3)	Bruner SD, et al. (1998) Repair of oxidatively damaged guanine in Saccharomyces cerevisiae by an alternative pathway. Curr Biol 8(7):393-403
4)	Alseth I, et al. (1999) The Saccharomyces cerevisiae homologues of endonuclease III from Escherichia coli, Ntg1 and Ntg2, are both required for efficient repair of spontaneous and induced oxidative DNA damage in yeast. Mol Cell Biol 19(5):3779-87
5)	Meadows KL, et al. (2003) Characterization of AP lyase activities of Saccharomyces cerevisiae Ntg1p and Ntg2p: implications for biological function. Nucleic Acids Res 31(19):5560-7
6)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7)	Griffiths LM, et al. (2009) Dynamic compartmentalization of base excision repair proteins in response to nuclear and mitochondrial oxidative stress. Mol Cell Biol 29(3):794-807
8)	Hori A, et al. (2009) Reactive oxygen species regulate DNA copy number in isolated yeast mitochondria by triggering recombination-mediated replication. Nucleic Acids Res 37(3):749-61