NSE4/YDL105W Summary

NSE4/YDL105W Summary

Standard Name	NSE4 1
Systematic Name	YDL105W
Alias	QRI2 2
Feature Type	ORF, Verified
Description	Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair (1, 3, 4, 5)
Name Description	Non-SMC Element 1

Chromosomal Location	ChrIV:272389 to 273597 \| ORF Map \| GBrowse

Gene Ontology Annotations All NSE4 GO evidence and references

	View Computational GO annotations for NSE4
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	DNA repair (IMP, IPI)
Cellular Component
Manually curated	Smc5-Smc6 complex (IDA)
High-throughput	nucleus (IDA)

Mutant phenotypes All NSE4 Phenotype evidence and references

Classical genetics
conditional	chromosome/plasmid maintenance: abnormal colony sectoring: increased
Large-scale survey
conditional	chromosome/plasmid maintenance: abnormal heat sensitivity: increased resistance to hydroxyurea: decreased resistance to methyl methanesulfonate: decreased viability: decreased
null	inviable resistance to tenovin-6: decreased
reduction of function	competitive fitness: increased
Resources	PROPHECY \| PhenoM \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All NSE4 Interaction evidence and references

	30 total interaction(s) for 12 unique genes/features.
Physical Interactions	Affinity Capture-MS: 9 Co-purification: 2 Reconstituted Complex: 3 Two-hybrid: 11
Genetic Interactions	Dosage Rescue: 3 Positive Genetic: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Mass Spec \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All NSE4 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:272389 to 273597 | |

SGD ORF map

Last Update

Coordinates: 2004-02-11 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1209	272389..273597	2004-02-11	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000002263

References cited on this page View Complete Literature Guide for NSE4

1)	Hu B, et al. (2005) Qri2/Nse4, a component of the essential Smc5/6 DNA repair complex. Mol Microbiol 55(6):1735-50
2)	Simon M, et al. (1994) Sequence of the PHO2-POL3 (CDC2) region of chromosome IV of Saccharomyces cerevisiae. Yeast 10(12):1653-6
3)	Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
4)	Zhao X and Blobel G (2005) A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organization. Proc Natl Acad Sci U S A 102(13):4777-82
5)	Bermudez-Lopez M, et al. (2010) The Smc5/6 complex is required for dissolution of DNA-mediated sister chromatid linkages. Nucleic Acids Res 38(19):6502-12