NRD1/YNL251C Summary Help

Standard Name NRD1 1
Systematic Name YNL251C
Feature Type ORF, Verified
Description RNA-binding subunit of Nrd1 complex; complex interacts with exosome to mediate 3'-end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with CTD of RNA pol II large subunit Rpo21p at phosphorylated Ser5 to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites; role in regulation of mitochondrial abundance and cell size (2, 3, 4, 5, 6, 7)
Name Description Nuclear pre-mRNA Down-regulation 1
Chromosomal Location
ChrXIV:174315 to 172588 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All NRD1 GO evidence and references
  View Computational GO annotations for NRD1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
reduction of function
Large-scale survey
reduction of function
321 total interaction(s) for 251 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 65
  • Affinity Capture-RNA: 74
  • Affinity Capture-Western: 10
  • Co-crystal Structure: 2
  • Co-localization: 4
  • Protein-peptide: 3
  • Reconstituted Complex: 3
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 74
  • Phenotypic Enhancement: 4
  • Positive Genetic: 10
  • Synthetic Growth Defect: 59
  • Synthetic Lethality: 4
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 575
Molecular Weight (Da) 63,858
Isoelectric Point (pI) 9.96
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIV:174315 to 172588 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1728 174315..172588 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005195
References cited on this page View Complete Literature Guide for NRD1
1) Steinmetz EJ and Brow DA  (1996) Repression of gene expression by an exogenous sequence element acting in concert with a heterogeneous nuclear ribonucleoprotein-like protein, Nrd1, and the putative helicase Sen1. Mol Cell Biol 16(12):6993-7003
2) Conrad NK, et al.  (2000) A yeast heterogeneous nuclear ribonucleoprotein complex associated with RNA polymerase II. Genetics 154(2):557-71
3) Steinmetz EJ and Brow DA  (1998) Control of pre-mRNA accumulation by the essential yeast protein Nrd1 requires high-affinity transcript binding and a domain implicated in RNA polymerase II association. Proc Natl Acad Sci U S A 95(12):6699-704
4) Steinmetz EJ, et al.  (2001) RNA-binding protein Nrd1 directs poly(A)-independent 3'-end formation of RNA polymerase II transcripts. Nature 413(6853):327-31
5) Vasiljeva L, et al.  (2008) The Nrd1-Nab3-Sen1 termination complex interacts with the Ser5-phosphorylated RNA polymerase II C-terminal domain. Nat Struct Mol Biol 15(8):795-804
6) Terzi N, et al.  (2011) H3K4 trimethylation by Set1 promotes efficient termination by the Nrd1-Nab3-Sen1 pathway. Mol Cell Biol 31(17):3569-83
7) Darby MM, et al.  (2012) The Saccharomyces cerevisiae Nrd1-Nab3 transcription termination pathway acts in opposition to Ras signaling and mediates response to nutrient depletion. Mol Cell Biol 32(10):1762-75