NPR2/YEL062W Summary

NPR2/YEL062W Summary

Standard Name	NPR2 1
Systematic Name	YEL062W
Feature Type	ORF, Verified
Description	Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline (1, 2, 3, 4, 5, 6)
Name Description	Nitrogen Permease Regulator 1

Chromosomal Location	ChrV:34407 to 36254 \| ORF Map \| GBrowse

Gene Ontology Annotations All NPR2 GO evidence and references

	View Computational GO annotations for NPR2
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	cellular response to amino acid starvation (IMP) cellular response to nitrogen starvation (IMP) proline transport (IMP) regulation of TOR signaling cascade (IMP) response to drug (IMP) urea transport (IMP)
Cellular Component
Manually curated	extrinsic to fungal-type vacuolar membrane (IDA) Seh1-associated complex (IDA)

Mutant phenotypes All NPR2 Phenotype evidence and references

Classical genetics
null	autophagy: decreased tellurium atom accumulation: increased metal resistance: decreased mutation frequency: increased Gat1p distribution: abnormal resistance to cisplatin: increased resistance to doxorubicin: increased resistance to tellurite: decreased sporulation efficiency: decreased utilization of nitrogen source: decreased
unspecified	resistance to cisplatin: increased
Large-scale survey
null	acid pH resistance: decreased glycogen accumulation: decreased glutathione excretion: increased competitive fitness: decreased dessication resistance: decreased metal resistance: decreased resistance to sodium arsenite: increased resistance to bleomycin: increased resistance to arsenite(3-): decreased resistance to tunicamycin: decreased resistance to 1,4-dithiothreitol: decreased resistance to camptothecin: decreased resistance to cycloheximide: decreased resistance to fluconazole: decreased resistance to mycophenolic acid: decreased resistance to caffeine: increased resistance to L-1,4-dithiothreitol: decreased sporulation: absent sporulation efficiency: decreased stress resistance: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All NPR2 Interaction evidence and references

	39 total interaction(s) for 25 unique genes/features.
Physical Interactions	Affinity Capture-MS: 18 Affinity Capture-Western: 6 Two-hybrid: 2
Genetic Interactions	Dosage Lethality: 1 Negative Genetic: 4 Synthetic Growth Defect: 2 Synthetic Lethality: 6
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All NPR2 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrV:34407 to 36254 | |

SGD ORF map

Last Update

Coordinates: 2003-09-22 | Sequence: 2003-09-22

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1848	34407..36254	2003-09-22	2003-09-22

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000788

References cited on this page View Complete Literature Guide for NPR2

1)	Rousselet G, et al. (1995) A second nitrogen permease regulator in Saccharomyces cerevisiae. FEBS Lett 359(2-3):215-9
2)	Schenk PW, et al. (2003) Anticancer drug resistance induced by disruption of the Saccharomyces cerevisiae NPR2 gene: a novel component involved in cisplatin- and doxorubicin-provoked cell kill. Mol Pharmacol 64(2):259-68
3)	Neklesa TK and Davis RW (2009) A Genome-Wide Screen for Regulators of TORC1 in Response to Amino Acid Starvation Reveals a Conserved Npr2/3 Complex. PLoS Genet 5(6):e1000515
4)	Spielewoy N, et al. (2010) Npr2, yeast homolog of the human tumor suppressor NPRL2, is a target of Grr1 required for adaptation to growth on diverse nitrogen sources. Eukaryot Cell 9(4):592-601
5)	Dokudovskaya S, et al. (2011) A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae. Mol Cell Proteomics 10(6):M110.006478
6)	Wu X and Tu BP (2011) Selective regulation of autophagy by the Iml1-Npr2-Npr3 complex in the absence of nitrogen starvation. Mol Biol Cell 22(21):4124-33