NPR2/YEL062W Summary Help

Standard Name NPR2 1
Systematic Name YEL062W
Feature Type ORF, Verified
Description Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human tumor suppressor NPRL2; target of Grr1p; required for growth on urea and proline (1, 2, 3, 4, 5, 6)
Name Description Nitrogen Permease Regulator 1
Chromosomal Location
ChrV:34407 to 36254 | ORF Map | GBrowse
Gene Ontology Annotations All NPR2 GO evidence and references
  View Computational GO annotations for NPR2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 59 genes
Classical genetics
Large-scale survey
39 total interaction(s) for 25 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 18
  • Affinity Capture-Western: 6
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 4
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 6

Expression Summary
Length (a.a.) 615
Molecular Weight (Da) 69,858
Isoelectric Point (pI) 8.62
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrV:34407 to 36254 | ORF Map | GBrowse
Last Update Coordinates: 2003-09-22 | Sequence: 2003-09-22
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1848 34407..36254 2003-09-22 2003-09-22
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000788
References cited on this page View Complete Literature Guide for NPR2
1) Rousselet G, et al.  (1995) A second nitrogen permease regulator in Saccharomyces cerevisiae. FEBS Lett 359(2-3):215-9
2) Schenk PW, et al.  (2003) Anticancer drug resistance induced by disruption of the Saccharomyces cerevisiae NPR2 gene: a novel component involved in cisplatin- and doxorubicin-provoked cell kill. Mol Pharmacol 64(2):259-68
3) Neklesa TK and Davis RW  (2009) A Genome-Wide Screen for Regulators of TORC1 in Response to Amino Acid Starvation Reveals a Conserved Npr2/3 Complex. PLoS Genet 5(6):e1000515
4) Spielewoy N, et al.  (2010) Npr2, yeast homolog of the human tumor suppressor NPRL2, is a target of Grr1 required for adaptation to growth on diverse nitrogen sources. Eukaryot Cell 9(4):592-601
5) Dokudovskaya S, et al.  (2011) A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae. Mol Cell Proteomics 10(6):M110.006478
6) Wu X and Tu BP  (2011) Selective regulation of autophagy by the Iml1-Npr2-Npr3 complex in the absence of nitrogen starvation. Mol Biol Cell 22(21):4124-33