Standard Name	NPL4 1
Systematic Name	YBR170C
Alias	HRD4
Feature Type	ORF, Verified
Description	Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP) (2, 3, 4, 5, 6, 7)
Name Description	Nuclear Protein Localization 1

Chromosomal Location	ChrII:578086 to 576344 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for NPL4
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	cytoplasm-associated proteasomal ubiquitin-dependent protein catabolic process (IMP) ER-associated misfolded protein catabolic process (IMP) ER-associated protein catabolic process (IMP) mitochondria-associated protein catabolic process (IMP) mitotic spindle disassembly (IMP) positive regulation of protein localization to nucleus (IMP) proteasomal ubiquitin-dependent protein catabolic process (IMP) retrograde protein transport, ER to cytosol (IMP)
Cellular Component
Manually curated	Cdc48p-Npl4p-Ufd1p AAA ATPase complex (IDA) Cdc48p-Npl4p-Vms1p AAA ATPase complex (IDA) Doa10p ubiquitin ligase complex (IDA) nuclear outer membrane-endoplasmic reticulum membrane network (IDA)
High-throughput	cytoplasm (IDA) integral to membrane (ISM) nucleus (IDA)

Classical genetics
conditional	cell cycle progression in G1 phase: delayed heat sensitivity: increased nuclear import: decreased nuclear morphology: abnormal Fbp1p accumulation: increased CPY* accumulation: increased Fzo1p accumulation: increased Ura3p-CL1 accumulation: increased Cdc48p distribution: abnormal CPY* distribution: abnormal myc-spt23p distribution: abnormal GFP-Spt23p distribution: abnormal GFP-Mga2p modification: decreased respiratory growth: decreased spindle morphology: abnormal toxin resistance: decreased
Large-scale survey
null	competitive fitness: decreased haploinsufficient resistance to cycloheximide: increased resistance to bleomycin: decreased resistance to camptothecin: decreased resistance to caffeine: decreased resistance to fluconazole: decreased resistance to methyl methanesulfonate: decreased resistance to clioquinol: decreased resistance to acetic acid: increased viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	108 total interaction(s) for 48 unique genes/features.
Physical Interactions	Affinity Capture-MS: 39 Affinity Capture-RNA: 1 Affinity Capture-Western: 22 Biochemical Activity: 2 Co-purification: 2 Reconstituted Complex: 2 Two-hybrid: 5
Genetic Interactions	Dosage Rescue: 5 Negative Genetic: 1 Phenotypic Suppression: 4 Positive Genetic: 1 Synthetic Growth Defect: 3 Synthetic Lethality: 5 Synthetic Rescue: 16
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrII:578086 to 576344 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1743 578086..576344 2011-02-03 1997-01-28
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000374