NPL3/YDR432W Summary 
| Standard Name | NPL3 1 |
|---|---|
| Systematic Name | YDR432W |
| Alias | MTR13 2 , MTS1 3 , NOP3 4 , NAB1 5 |
| Feature Type | ORF, Verified |
| Description | RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress (6, 7, 8, 9, 10, 11, 12) |
| Name Description | Nuclear Protein Localization 1 |
| Chromosomal Location | |
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Gene Ontology Annotations All NPL3 GO evidence and references
| View Computational GO annotations for NPL3 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated |
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| Cellular Component | |
| Manually curated | |
| High-throughput |
Mutant phenotypes All NPL3 Phenotype evidence and references
interactions All NPL3 Interaction evidence and references
| 1325 total interaction(s) for 1102 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| Resources |
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Expression Summary
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| Resources |
Protein Information All NPL3 Protein evidence and references
| Localization | |
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| Phosphorylation | PhosphoGRID | PhosphoPep Database |
| Structure | |
| Homologs |
sequence information
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| Last Update | Coordinates: 2011-02-03 | Sequence: 1996-07-31 | ||||||||||||
| Subfeature details |
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Analyze Sequence
| S288C only | |
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| S288C vs. other species | |
| S288C vs. other strains |
Resources
| External Links | All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB |
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| Primary SGDID | S000002840 |
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References cited on this page View Complete Literature Guide for NPL3
| 1) | Bossie MA, et al. (1992) A mutant nuclear protein with similarity to RNA binding proteins interferes with nuclear import in yeast. Mol Biol Cell 3(8):875-93 |
| 2) | Kadowaki T, et al. (1994) Isolation and characterization of Saccharomyces cerevisiae mRNA transport-defective (mtr) mutants. J Cell Biol 126(3):649-59 |
| 3) | Ellis EM and Reid GA (1993) The Saccharomyces cerevisiae MTS1 gene encodes a putative RNA-binding protein involved in mitochondrial protein targeting. Gene 132(2):175-83 |
| 4) | Russell ID and Tollervey D (1992) NOP3 is an essential yeast protein which is required for pre-rRNA processing. J Cell Biol 119(4):737-47 |
| 5) | Wilson SM, et al. (1994) Characterization of nuclear polyadenylated RNA-binding proteins in Saccharomyces cerevisiae. J Cell Biol 127(5):1173-84 |
| 6) | Lee MS, et al. (1996) A protein that shuttles between the nucleus and the cytoplasm is an important mediator of RNA export. Genes Dev 10(10):1233-46 |
| 7) | Gilbert W, et al. (2001) Phosphorylation by Sky1p promotes Npl3p shuttling and mRNA dissociation. RNA 7(2):302-13 |
| 8) | Windgassen M, et al. (2004) Yeast shuttling SR proteins Npl3p, Gbp2p, and Hrb1p are part of the translating mRNPs, and Npl3p can function as a translational repressor. Mol Cell Biol 24(23):10479-91 |
| 9) | Dermody JL, et al. (2008) Unphosphorylated SR-like protein Npl3 stimulates RNA polymerase II elongation. PLoS ONE 3(9):e3273 |
| 10) | Kress TL, et al. (2008) A Single SR-like Protein, Npl3, Promotes Pre-mRNA Splicing in Budding Yeast. Mol Cell 32(5):727-34 |
| 11) | Rajyaguru P, et al. (2012) Scd6 Targets eIF4G to Repress Translation: RGG Motif Proteins as a Class of eIF4G-Binding Proteins. Mol Cell 45(2):244-54 |
| 12) | Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76 |
