NOP56/YLR197W Summary Help

Standard Name NOP56 1
Systematic Name YLR197W
Alias SIK1 2
Feature Type ORF, Verified
Description Essential evolutionarily-conserved nucleolar protein; component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects (1, 3, 4, 5)
Name Description NucleOlar Protein of 56.8 kDa 1
Chromosomal Location
ChrXII:546097 to 547611 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All NOP56 GO evidence and references
  View Computational GO annotations for NOP56
Molecular Function
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 8 genes
Resources
Classical genetics
null
overexpression
repressible
Large-scale survey
conditional
null
reduction of function
Resources
236 total interaction(s) for 165 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 155
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 11
  • PCA: 1
  • Two-hybrid: 10

Genetic Interactions
  • Dosage Lethality: 3
  • Negative Genetic: 17
  • Positive Genetic: 29
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 504
Molecular Weight (Da) 56,864
Isoelectric Point (pI) 9.63
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:546097 to 547611 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1515 546097..547611 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004187
References cited on this page View Complete Literature Guide for NOP56
1) Gautier T, et al.  (1997) Nucleolar KKE/D repeat proteins Nop56p and Nop58p interact with Nop1p and are required for ribosome biogenesis. Mol Cell Biol 17(12):7088-98
2) Morin PJ, et al.  (1995) Genetic analysis of growth inhibition by GAL4-L kappa B-alpha in Saccharomyces cerevisiae. Cell Growth Differ 6(7):789-98
3) Lafontaine DL and Tollervey D  (2000) Synthesis and assembly of the box C+D small nucleolar RNPs. Mol Cell Biol 20(8):2650-9
4) Bogomolnaya LM, et al.  (2004) A new enrichment approach identifies genes that alter cell cycle progression in Saccharomyces cerevisiae. Curr Genet 45(6):350-9
5) Hayano T, et al.  (2003) Proteomic analysis of human Nop56p-associated pre-ribosomal ribonucleoprotein complexes. Possible link between Nop56p and the nucleolar protein treacle responsible for Treacher Collins syndrome. J Biol Chem 278(36):34309-19