NOP1/YDL014W Summary Help

Standard Name NOP1 1, 2
Systematic Name YDL014W
Alias LOT3
Feature Type ORF, Verified
Description Histone glutamine methyltransferase, modifies H2A at Q105 in nucleolus; component of the small subunit processome complex, which is required for processing of pre-18S rRNA; ortholog of mammalian fibrillarin (1, 3, 4, 5)
Name Description NucleOlar Protein 1, 2
Chromosomal Location
ChrIV:427364 to 428347 | ORF Map | GBrowse
Gbrowse
Genetic position: -1.93 cM
Gene Ontology Annotations All NOP1 GO evidence and references
  View Computational GO annotations for NOP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
repressible
Large-scale survey
null
overexpression
reduction of function
Resources
414 total interaction(s) for 290 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 256
  • Affinity Capture-RNA: 6
  • Affinity Capture-Western: 29
  • Biochemical Activity: 1
  • Co-localization: 7
  • Co-purification: 2
  • PCA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 9

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 18
  • Positive Genetic: 39
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 27
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 327
Molecular Weight (Da) 34,465
Isoelectric Point (pI) 11.03
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:427364 to 428347 | ORF Map | GBrowse
SGD ORF map
Genetic position: -1.93 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..984 427364..428347 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002172
References cited on this page View Complete Literature Guide for NOP1
1) Schimmang T, et al.  (1989) A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability. EMBO J 8(13):4015-24
2) Henriquez R, et al.  (1990) Isolation and sequencing of NOP1. A yeast gene encoding a nucleolar protein homologous to a human autoimmune antigen. J Biol Chem 265(4):2209-15
3) Tollervey D, et al.  (1991) The small nucleolar RNP protein NOP1 (fibrillarin) is required for pre-rRNA processing in yeast. EMBO J 10(3):573-83
4) Dragon F, et al.  (2002) A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis. Nature 417(6892):967-70
5) Tessarz P, et al.  (2014) Glutamine methylation in histone H2A is an RNA-polymerase-I-dedicated modification. Nature 505(7484):564-8