Standard Name	NOP1
Systematic Name	YDL014W
Alias	LOT3
Feature Type	ORF, Verified
Description	Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin (1, 2, 3)
Name Description	NucleOlar Protein 2, 4

Chromosomal Location	ChrIV:427364 to 428347 | ORF Map | GBrowse
	Genetic position: -1.93 cM

	View Computational GO annotations for NOP1
Molecular Function
Manually curated	rRNA methyltransferase activity (IDA, IMP)
Biological Process
Manually curated	box C/D snoRNA 3'-end processing (IMP) rRNA methylation (IDA, IMP) rRNA processing (IMP) snoRNA processing (IMP)
Cellular Component
Manually curated	box C/D snoRNP complex (IDA) nucleolus (IDA) small-subunit processome (IDA)
High-throughput	90S preribosome (IDA) colocalizes_with ribosome (IDA)

Classical genetics
null	inviable
repressible	35S pre-rRNA accumulation: increased mature 18S rRNA accumulation: absent 20S rRNA processing intermediate accumulation: absent 27SA rRNA processing intermediate accumulation: absent 32S rRNA processing intermediate accumulation: absent aberrant 23S rRNA processing intermediate accumulation: increased snR52 precursor accumulation: increased box C/D family U14 snoRNA localization: abnormal box C/D snoRNA construct derived from U14 (snR128) localization: abnormal 40S ribosomal subunit accumulation: decreased
Large-scale survey
null	cell size: decreased haploinsufficient heat sensitivity: increased inviable
overexpression	cell cycle progression: abnormal vegetative growth: decreased rate
reduction of function	competitive fitness: decreased resistance to tunicamycin: decreased
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	406 total interaction(s) for 285 unique genes/features.
Physical Interactions	Affinity Capture-MS: 254 Affinity Capture-RNA: 5 Affinity Capture-Western: 28 Biochemical Activity: 1 Co-localization: 6 Co-purification: 2 Reconstituted Complex: 1 Two-hybrid: 9
Genetic Interactions	Dosage Rescue: 4 Negative Genetic: 18 Positive Genetic: 39 Synthetic Growth Defect: 12 Synthetic Lethality: 27
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Mass Spec

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIV:427364 to 428347 | ORF Map | GBrowse
	Genetic position: -1.93 cM
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..984 427364..428347 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000002172