NOB1/YOR056C Summary Help

Standard Name NOB1 1
Systematic Name YOR056C
Alias YOR29-07
Feature Type ORF, Verified
Description Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress (1, 2, 3, 4, 5)
Name Description Nin1 (One) Binding protein 1
Chromosomal Location
ChrXV:431626 to 430247 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NOB1 GO evidence and references
  View Computational GO annotations for NOB1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Classical genetics
null
repressible
Large-scale survey
conditional
null
reduction of function
Resources
106 total interaction(s) for 78 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 48
  • Affinity Capture-Western: 5
  • Biochemical Activity: 4
  • Co-crystal Structure: 1
  • Co-fractionation: 2
  • Co-purification: 2
  • Protein-peptide: 2
  • Reconstituted Complex: 9
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 27
  • Synthetic Lethality: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 459
Molecular Weight (Da) 51,742
Isoelectric Point (pI) 8.65
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:431626 to 430247 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1380 431626..430247 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005582
References cited on this page View Complete Literature Guide for NOB1
1) Tone Y, et al.  (2000) Nob1p, a new essential protein, associates with the 26S proteasome of growing saccharomyces cerevisiae cells. Gene 243(1-2):37-45
2) Tone Y and Toh-E A  (2002) Nob1p is required for biogenesis of the 26S proteasome and degraded upon its maturation in Saccharomyces cerevisiae. Genes Dev 16(24):3142-57
3) Fatica A, et al.  (2003) Nob1p is required for cleavage of the 3' end of 18S rRNA. Mol Cell Biol 23(5):1798-807
4) Lebaron S, et al.  (2012) Proofreading of pre-40S ribosome maturation by a translation initiation factor and 60S subunits. Nat Struct Mol Biol 19(8):744-53
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76