NMD2/YHR077C Summary

NMD2/YHR077C Summary

Standard Name	NMD2 1
Systematic Name	YHR077C
Alias	IFS1 , SUA1 2 , UPF2 3 , SUP111 4
Feature Type	ORF, Verified
Description	Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance (1, 3, 5, 6)
Name Description	Nonsense-mediated MRNA Decay 1

Chromosomal Location	ChrVIII:255756 to 252374 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

	Genetic position: 51 cM

Gene Ontology Annotations All NMD2 GO evidence and references

	View Computational GO annotations for NMD2
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay (IGI) NOT translational frameshifting (IMP)
Cellular Component
Manually curated	cytoplasm (IDA) polysome (IDA)

Mutant phenotypes All NMD2 Phenotype evidence and references

Classical genetics
null	PGK1(N103)pG mRNA accumulation: increased Upf3p distribution: abnormal Dcp2p distribution: abnormal
Large-scale survey
null	competitive fitness: decreased ionic stress resistance: decreased resistance to fenpropimorph: increased resistance to bleomycin: increased resistance to sodium arsenite: increased resistance to cisplatin: increased resistance to fluconazole: increased resistance to tunicamycin: increased resistance to camptothecin: decreased resistance to cycloheximide: decreased resistance to L-canavanine: increased resistance to caffeine: increased telomere length: decreased transposable element transposition: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All NMD2 Interaction evidence and references

	203 total interaction(s) for 157 unique genes/features.
Physical Interactions	Affinity Capture-MS: 11 Affinity Capture-RNA: 1 Affinity Capture-Western: 6 Reconstituted Complex: 1 Two-hybrid: 11
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 2 Negative Genetic: 99 Phenotypic Enhancement: 4 Phenotypic Suppression: 2 Positive Genetic: 44 Synthetic Growth Defect: 7 Synthetic Lethality: 3 Synthetic Rescue: 11
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All NMD2 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVIII:255756 to 252374 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

: 51 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1999-11-17

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..6	255756..255751	2011-02-03	1999-11-17
Intron	7..119	255750..255638	2011-02-03	1999-11-17
CDS	120..3383	255637..252374	2011-02-03	1999-11-17

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001119

References cited on this page View Complete Literature Guide for NMD2

1)	He F and Jacobson A (1995) Identification of a novel component of the nonsense-mediated mRNA decay pathway by use of an interacting protein screen. Genes Dev 9(4):437-54
2)	Hampsey M, et al. (1991) Extragenic suppressors of a translation initiation defect in the cyc1 gene of Saccharomyces cerevisiae. Biochimie 73(12):1445-55
3)	Cui Y, et al. (1995) Identification and characterization of genes that are required for the accelerated degradation of mRNAs containing a premature translational termination codon. Genes Dev 9(4):423-36
4)	Ono B, et al. (2005) Suppression of termination mutations caused by defects of the NMD machinery in Saccharomyces cerevisiae. Genes Genet Syst 80(5):311-6
5)	He F, et al. (1997) Upf1p, Nmd2p, and Upf3p are interacting components of the yeast nonsense-mediated mRNA decay pathway. Mol Cell Biol 17(3):1580-94
6)	Askree SH, et al. (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63