Standard Name	NIP100 1
Systematic Name	YPL174C
Alias	PAC13 2
Feature Type	ORF, Verified
Description	Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) (1, 3)
Name Description	Nuclear ImPort

Chromosomal Location	ChrXVI:222773 to 220167 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for NIP100
Molecular Function
Manually curated	microtubule binding (ISS)
Biological Process
Manually curated	establishment of mitotic spindle orientation (IMP)
Cellular Component
Manually curated	colocalizes_with astral microtubule (IDA) colocalizes_with cell cortex (IDA) dynactin complex (IDA) colocalizes_with spindle pole body (IDA)

Classical genetics
null	spindle morphology: abnormal
overexpression	nuclear morphology: abnormal
Large-scale survey
null	auxotrophy cell cycle progression in G1 phase: increased duration competitive fitness: decreased dessication resistance: decreased haploinsufficient heat sensitivity: increased resistance to arsenite(3-): decreased resistance to sulfometuron methyl: decreased resistance to mycophenolic acid: increased resistance to methyl methanesulfonate: increased resistance to fluconazole: increased resistance to bleomycin: increased resistance to cycloheximide: decreased resistance to ethanol: decreased resistance to tunicamycin: decreased resistance to camptothecin: decreased respiratory growth: absent respiratory growth: decreased rate stress resistance: decreased transposable element transposition: decreased utilization of carbon source: decreased vacuolar morphology: abnormal vegetative growth: decreased rate viable
overexpression	cell cycle progression: abnormal vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	449 total interaction(s) for 278 unique genes/features.
Physical Interactions	Affinity Capture-MS: 4 Affinity Capture-Western: 18 Co-fractionation: 1 PCA: 2 Reconstituted Complex: 1 Two-hybrid: 122
Genetic Interactions	Negative Genetic: 189 Phenotypic Enhancement: 2 Positive Genetic: 24 Synthetic Growth Defect: 48 Synthetic Lethality: 38
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXVI:222773 to 220167 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2607 222773..220167 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000006095