NIC96/YFR002W Summary Help

Standard Name NIC96 1
Systematic Name YFR002W
Feature Type ORF, Verified
Description Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) (1, 2, 3, 4, 5, 6, 7, 8, 9 and see Summary Paragraph)
Name Description Nucleoporin-Interacting Component of 96 kDa 1
Chromosomal Location
ChrVI:150016 to 152535 | ORF Map | GBrowse
Gene Ontology Annotations All NIC96 GO evidence and references
  View Computational GO annotations for NIC96
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
Large-scale survey
reduction of function
148 total interaction(s) for 77 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 52
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 16
  • Co-purification: 5
  • Far Western: 1
  • FRET: 5
  • PCA: 3
  • Protein-peptide: 1
  • Reconstituted Complex: 7
  • Two-hybrid: 13

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 2
  • Negative Genetic: 33
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 5

Expression Summary
Length (a.a.) 839
Molecular Weight (Da) 96,173
Isoelectric Point (pI) 6.81
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVI:150016 to 152535 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2520 150016..152535 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001898

NIC96 encodes an abundant, essential nuclear pore protein (1, 10, 11). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 11, 12, 13, 14). The structure of the vertebrate NPC has been studied extensively; recent reviews include 15, 16, 17, and 18. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (19, 20). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 11, 12, 21, 22, 23). Nic96p is found in a subcomplex of the NPC, often called the Nsp1p subcomplex, that also contains Nsp1p, Nup49p, and Nup57p (11, 6, 1). This complex has been detected at both the nuclear and cytoplasmic periphery of the NPC central channel (2). Nic96p and the other most abundant nucleoporins, Pom152p, Nup157p, Nup170p, and Nup188p, may form the structural core of the NPC (11). Mutations in NIC96 cause defects in nuclear protein import (11); nic96 mutations are also synthetically lethal with mutations in other nucleoporin genes (24, 11). Proteins related to Nic96p have been identified in several other species, including S. pombe (25), C. elegans (26), and vertebrates (26, 27).

Last updated: 1999-08-05 Contact SGD

References cited on this page View Complete Literature Guide for NIC96
1) Grandi P, et al.  (1993) Purification of NSP1 reveals complex formation with 'GLFG' nucleoporins and a novel nuclear pore protein NIC96. EMBO J 12(8):3061-71
2) Fahrenkrog B, et al.  (1998) Molecular architecture of the yeast nuclear pore complex: localization of Nsp1p subcomplexes. J Cell Biol 143(3):577-88
3) Rout MP, et al.  (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 148(4):635-51
4) Grandi P, et al.  (1995) Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p. EMBO J 14(1):76-87
5) Zabel U, et al.  (1996) Nic96p is required for nuclear pore formation and functionally interacts with a novel nucleoporin, Nup188p. J Cell Biol 133(6):1141-52
6) Schlaich NL, et al.  (1997) In vitro reconstitution of a heterotrimeric nucleoporin complex consisting of recombinant Nsp1p, Nup49p, and Nup57p. Mol Biol Cell 8(1):33-46
7) Bailer SM, et al.  (2001) The Nsp1p carboxy-terminal domain is organized into functionally distinct coiled-coil regions required for assembly of nucleoporin subcomplexes and nucleocytoplasmic transport. Mol Cell Biol 21(23):7944-55
8) Lutzmann M, et al.  (2005) Reconstitution of Nup157 and Nup145N into the Nup84 complex. J Biol Chem 280(18):18442-51
9) Alber F, et al.  (2007) The molecular architecture of the nuclear pore complex. Nature 450(7170):695-701
10) Aitchison JD, et al.  (1995) Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p. J Cell Biol 131(5):1133-48
11) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
12) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
13) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
14) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
15) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
16) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
17) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
18) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
19) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
20) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
21) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
22) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
23) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
24) Tcheperegine SE, et al.  (1999) Topology and functional domains of the yeast pore membrane protein Pom152p. J Biol Chem 274(8):5252-8
25) Yoon JH, et al.  (1997) Npp106p, a Schizosaccharomyces pombe nucleoporin similar to Saccharomyces cerevisiae Nic96p, functionally interacts with Rae1p in mRNA export. Mol Cell Biol 17(12):7047-60
26) Grandi P, et al.  (1997) Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a novel 205-kDa protein and is required for correct nuclear pore assembly. Mol Biol Cell 8(10):2017-38
27) Allende ML, et al.  (1996) Insertional mutagenesis in zebrafish identifies two novel genes, pescadillo and dead eye, essential for embryonic development. Genes Dev 10(24):3141-55