NHP10/YDL002C Summary Help

Standard Name NHP10 1
Systematic Name YDL002C
Alias HMO2 2
Feature Type ORF, Verified
Description Non-essential INO80 chromatin remodeling complex subunit; preferentially binds DNA ends, protecting them from exonucleatic cleavage; deletion affects telomere maintenance via recombination; related to mammalian high mobility group proteins (2, 3, 4, 5, 6, 7)
Name Description Non-Histone Protein 8
Chromosomal Location
ChrIV:447578 to 446967 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NHP10 GO evidence and references
  View Computational GO annotations for NHP10
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
451 total interaction(s) for 320 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 77
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 2
  • Biochemical Activity: 9
  • Co-purification: 1
  • PCA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 11

Genetic Interactions
  • Dosage Growth Defect: 2
  • Negative Genetic: 277
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 1
  • Positive Genetic: 28
  • Synthetic Growth Defect: 30
  • Synthetic Lethality: 3
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 203
Molecular Weight (Da) 23,857
Isoelectric Point (pI) 8.06
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:447578 to 446967 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..612 447578..446967 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002160
References cited on this page View Complete Literature Guide for NHP10
1) Kasahara K, et al.  (2007) Assembly of Regulatory Factors on rRNA and Ribosomal Protein Genes in Saccharomyces cerevisiae. Mol Cell Biol 27(19):6686-6705
2) Lu J, et al.  (1996) Characterization of a high mobility group 1/2 homolog in yeast. J Biol Chem 271(52):33678-85
3) Shen X, et al.  (2003) Involvement of actin-related proteins in ATP-dependent chromatin remodeling. Mol Cell 12(1):147-55
4) Morrison AJ, et al.  (2004) INO80 and gamma-H2AX interaction links ATP-dependent chromatin remodeling to DNA damage repair. Cell 119(6):767-75
5) Conaway RC and Conaway JW  (2009) The INO80 chromatin remodeling complex in transcription, replication and repair. Trends Biochem Sci 34(2):71-7
6) Ray S and Grove A  (2009) The yeast high mobility group protein HMO2, a subunit of the chromatin-remodeling complex INO80, binds DNA ends. Nucleic Acids Res 37(19):6389-99
7) Hu Y, et al.  (2013) Telomerase-null survivor screening identifies novel telomere recombination regulators. PLoS Genet 9(1):e1003208
8) Kolodrubetz D, et al.  (1988) Amino-terminal sequence of a Saccharomyces cerevisiae nuclear protein, NHP6, shows significant identity to bovine HMG1. FEBS Lett 238(1):175-9
9) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87