NFI1/YOR156C Summary Help

Standard Name NFI1 1
Systematic Name YOR156C
Alias SIZ2 2
Feature Type ORF, Verified
Description SUMO E3 ligase; catalyzes sumoylation of Yku70p/Yku80p and Sir4p promoting chromatin anchoring; DNA-bound form catalyzes a DNA-damaged triggered sumoylation wave resulting in multisite modification of several DNA repair proteins, enhancing interactions between these proteins and accelerating repair; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length; NFI1 has a paralog, SIZ1, that arose from the whole genome duplication (3, 4, 5, 6, 7, 8)
Name Description Neck Filament Interacting 3
Chromosomal Location
ChrXV:630540 to 628360 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All NFI1 GO evidence and references
  View Computational GO annotations for NFI1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Classical genetics
Large-scale survey
205 total interaction(s) for 147 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 84
  • Affinity Capture-Western: 4
  • Biochemical Activity: 7
  • Reconstituted Complex: 1
  • Two-hybrid: 25

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 5
  • Phenotypic Enhancement: 18
  • Phenotypic Suppression: 9
  • Positive Genetic: 2
  • Synthetic Growth Defect: 38
  • Synthetic Lethality: 8
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 726
Molecular Weight (Da) 81,177
Isoelectric Point (pI) 7.23
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXV:630540 to 628360 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2181 630540..628360 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005682
References cited on this page View Complete Literature Guide for NFI1
1) de Virgilio, C. and J.R. Pringle  (1995) S. cerevisiae two-hybrid interactor with the C-terminus of Cdc12p. Unpublished
2) Strunnikov AV, et al.  (2001) Saccharomyces cerevisiae SMT4 encodes an evolutionarily conserved protease with a role in chromosome condensation regulation. Genetics 158(1):95-107
3) Johnson ES and Gupta AA  (2001) An E3-like factor that promotes SUMO conjugation to the yeast septins. Cell 106(6):735-44
4) Hochstrasser M  (2001) SP-RING for SUMO: new functions bloom for a ubiquitin-like protein. Cell 107(1):5-8
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Askree SH, et al.  (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63
7) Ferreira HC, et al.  (2011) The PIAS homologue Siz2 regulates perinuclear telomere position and telomerase activity in budding yeast.LID - 10.1038/ncb2263 [doi] Nat Cell Biol ()
8) Psakhye I and Jentsch S  (2012) Protein group modification and synergy in the SUMO pathway as exemplified in DNA repair. Cell 151(4):807-20