NEJ1/YLR265C Summary Help

Standard Name NEJ1 1
Systematic Name YLR265C
Alias LIF2 2
Feature Type ORF, Verified
Description Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p (1, 2, 3, 4)
Name Description Nonhomologous End-Joining defective 1
Chromosomal Location
ChrXII:675455 to 674427 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NEJ1 GO evidence and references
  View Computational GO annotations for NEJ1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
50 total interaction(s) for 34 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 9
  • Biochemical Activity: 6
  • Co-fractionation: 2
  • Co-purification: 1
  • Two-hybrid: 10

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 8
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 342
Molecular Weight (Da) 39,115
Isoelectric Point (pI) 7.97
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:675455 to 674427 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1029 675455..674427 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004255
References cited on this page View Complete Literature Guide for NEJ1
1) Kegel A, et al.  (2001) Nej1p, a cell type-specific regulator of nonhomologous end joining in yeast. Curr Biol 11(20):1611-7
2) Frank-Vaillant M and Marcand S  (2001) NHEJ regulation by mating type is exercised through a novel protein, Lif2p, essential to the ligase IV pathway. Genes Dev 15(22):3005-12
3) Valencia M, et al.  (2001) NEJ1 controls non-homologous end joining in Saccharomyces cerevisiae. Nature 414(6864):666-9
4) Deshpande RA and Wilson TE  (2007) Modes of interaction among yeast Nej1, Lif1 and Dnl4 proteins and comparison to human XLF, XRCC4 and Lig4. DNA Repair (Amst) 6(10):1507-16