Standard Name	NEJ1 1
Systematic Name	YLR265C
Alias	LIF2 2
Feature Type	ORF, Verified
Description	Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p (1, 2, 3, 4)
Name Description	Nonhomologous End-Joining defective 1

Chromosomal Location	ChrXII:675455 to 674427 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for NEJ1
Molecular Function
Manually curated	DNA end binding (IDA)
Biological Process
Manually curated	double-strand break repair via nonhomologous end joining (IMP) double-strand break repair via single-strand annealing (IMP)
Cellular Component
Manually curated	DNA ligase IV complex (IPI) nucleus (IDA)

Classical genetics
null	resistance to phleomycin: increased
Large-scale survey
null	competitive fitness: increased haploinsufficient resistance to acrolein: decreased resistance to cycloheximide: increased resistance to camptothecin: increased sporulation: normal viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	48 total interaction(s) for 32 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-RNA: 2 Affinity Capture-Western: 9 Biochemical Activity: 6 Co-fractionation: 2 Co-purification: 1 Two-hybrid: 10
Genetic Interactions	Dosage Rescue: 2 Negative Genetic: 8 Phenotypic Enhancement: 1 Phenotypic Suppression: 1 Positive Genetic: 1 Synthetic Growth Defect: 2 Synthetic Rescue: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXII:675455 to 674427 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1029 675455..674427 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000004255