Standard Name	NCS2 1, 2
Systematic Name	YNL119W
Alias	TUC2 3
Feature Type	ORF, Verified
Description	Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae (2, 3, 4)
Name Description	Needs Cla4 to Survive 1

Chromosomal Location	ChrXIV:401040 to 402521 | ORF Map | GBrowse

	View Computational GO annotations for NCS2
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	invasive growth in response to glucose limitation (IMP) protein urmylation (IMP) pseudohyphal growth (IMP) tRNA wobble position uridine thiolation (IMP)
Cellular Component
Manually curated	cytosol (IDA)
High-throughput	cytoplasm (IDA)

Classical genetics
null	tRNA modification: absent tRNA-Glu modification: absent growth in exponential phase: decreased rate heat sensitivity: increased resistance to sodium chloride: decreased resistance to caffeine: decreased resistance to canavanine: decreased viable
unspecified	viability: decreased
Large-scale survey
null	tRNA modification: abnormal auxotrophy competitive fitness: decreased dessication resistance: decreased heat sensitivity: increased oxidative stress resistance: decreased resistance to benzo[a]pyrene: increased resistance to sirolimus: decreased resistance to propolis extract: decreased resistance to quinine: decreased resistance to wortmannin: normal resistance to oleate: decreased resistance to hygromycin B: decreased sporulation: abnormal stress resistance: increased toxin resistance: increased vegetative growth: decreased rate viable
overexpression	filamentous growth: increased invasive growth: absent
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	97 total interaction(s) for 83 unique genes/features.
Physical Interactions	Affinity Capture-MS: 7 Affinity Capture-Western: 2 Two-hybrid: 3
Genetic Interactions	Dosage Rescue: 7 Negative Genetic: 51 Positive Genetic: 8 Synthetic Growth Defect: 11 Synthetic Lethality: 8
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXIV:401040 to 402521 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1482 401040..402521 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000005063