NCS2/YNL119W Summary Help

Standard Name NCS2 1, 2
Systematic Name YNL119W
Alias TUC2 3
Feature Type ORF, Verified
Description Protein required for uridine thiolation of Lys(UUU) and Glu(UUC) tRNAs; required for the thiolation of uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae (2, 3, 4)
Name Description Needs Cla4 to Survive 1
Chromosomal Location
ChrXIV:401040 to 402521 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All NCS2 GO evidence and references
  View Computational GO annotations for NCS2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
overexpression
Resources
102 total interaction(s) for 87 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Rescue: 7
  • Negative Genetic: 51
  • Positive Genetic: 9
  • Synthetic Growth Defect: 12
  • Synthetic Lethality: 8

Resources
Expression Summary
histogram
Resources
Length (a.a.) 493
Molecular Weight (Da) 56,472
Isoelectric Point (pI) 6.34
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:401040 to 402521 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1482 401040..402521 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005063
References cited on this page View Complete Literature Guide for NCS2
1) Goehring AS, et al.  (2003) Synthetic lethal analysis implicates Ste20p, a p21-activated potein kinase, in polarisome activation. Mol Biol Cell 14(4):1501-16
2) Goehring AS, et al.  (2003) Urmylation: a ubiquitin-like pathway that functions during invasive growth and budding in yeast. Mol Biol Cell 14(11):4329-41
3) Nakai Y, et al.  (2008) Thio-modification of Yeast Cytosolic tRNA Requires a Ubiquitin-related System That Resembles Bacterial Sulfur Transfer Systems. J Biol Chem 283(41):27469-76
4) Kushner DB, et al.  (2003) Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus. Proc Natl Acad Sci U S A 100(26):15764-9