NCL1/YBL024W Summary Help

Standard Name NCL1 1
Systematic Name YBL024W
Alias TRM4
Feature Type ORF, Verified
Description S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase; methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 (1, 2, 3)
Name Description NuCLear protein 1
Chromosomal Location
ChrII:172534 to 174588 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All NCL1 GO evidence and references
  View Computational GO annotations for NCL1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
overexpression
Resources
120 total interaction(s) for 97 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 14
  • Affinity Capture-RNA: 4
  • Biochemical Activity: 21
  • Co-purification: 1

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 23
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 7
  • Positive Genetic: 30
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 7
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 684
Molecular Weight (Da) 77,878
Isoelectric Point (pI) 7.32
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:172534 to 174588 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2055 172534..174588 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000120
References cited on this page View Complete Literature Guide for NCL1
1) Wu P, et al.  (1998) NCL1, a novel gene for a non-essential nuclear protein in Saccharomyces cerevisiae. Gene 220(1-2):109-17
2) Motorin Y and Grosjean H  (1999) Multisite-specific tRNA:m5C-methyltransferase (Trm4) in yeast Saccharomyces cerevisiae: identification of the gene and substrate specificity of the enzyme. RNA 5(8):1105-18
3) Chan CT, et al.  (2012) Reprogramming of tRNA modifications controls the oxidative stress response by codon-biased translation of proteins. Nat Commun 3():937