Standard Name	NCL1 1
Systematic Name	YBL024W
Alias	TRM4
Feature Type	ORF, Verified
Description	S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 (1, 2, 3)
Name Description	NuCLear protein 1

Chromosomal Location	ChrII:172534 to 174588 | ORF Map | GBrowse

	View Computational GO annotations for NCL1
Molecular Function
Manually curated	tRNA (cytosine-5-)-methyltransferase activity (IDA)
Biological Process
Manually curated	tRNA methylation (IDA)
Cellular Component
Manually curated	nucleus (IDA)

Classical genetics
null	tellurium atom accumulation: increased heat sensitivity: increased killer toxin resistance: decreased resistance to calcium dichloride: decreased resistance to tellurite: increased resistance to Papulacandin B: decreased
unspecified	transposable element transposition: decreased
Large-scale survey
null	cell size: increased competitive fitness: decreased oxidative stress resistance: decreased resistance to methyl methanesulfonate: decreased resistance to hydroxyurea: decreased resistance to fluconazole: decreased resistance to caffeine: decreased resistance to cycloheximide: decreased resistance to camptothecin: decreased resistance to sodium arsenite: decreased resistance to sirolimus: decreased resistance to benomyl: decreased resistance to ethanol: decreased resistance to tunicamycin: increased resistance to fenpropimorph: increased resistance to bleomycin: increased resistance to tetramethylammonium: increased resistance to spermine: increased resistance to hygromycin B: increased resistance to mycophenolic acid: decreased resistance to dimethyl sulfoxide: decreased resistance to sodium selenide: decreased stress resistance: increased vacuolar morphology: abnormal viable
overexpression	resistance to sirolimus: increased vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	114 total interaction(s) for 93 unique genes/features.
Physical Interactions	Affinity Capture-MS: 12 Affinity Capture-RNA: 2 Biochemical Activity: 21
Genetic Interactions	Dosage Rescue: 2 Negative Genetic: 22 Phenotypic Enhancement: 1 Phenotypic Suppression: 7 Positive Genetic: 30 Synthetic Growth Defect: 5 Synthetic Lethality: 7 Synthetic Rescue: 5
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrII:172534 to 174588 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2055 172534..174588 2011-02-03 1997-01-28
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000120