Standard Name	NCB2 1 (see Nomenclature conflict Note)
Systematic Name	YDR397C
Alias	YDR1
Feature Type	ORF, Verified
Description	Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta (1, 2, 3)
Name Description	Negative Cofactor B 1

Chromosomal Location	ChrIV:1266898 to 1266366 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for NCB2
Molecular Function
Manually curated	transcription coactivator activity (IDA) transcription corepressor activity (IGI, IMP)
Biological Process
Manually curated	negative regulation of transcription from RNA polymerase II promoter (IGI, IMP) positive regulation of transcription from RNA polymerase II promoter (IDA)
Cellular Component
Manually curated	negative cofactor 2 complex (IDA) nucleus (IPI)

Classical genetics
conditional	cold sensitivity: increased
null	inviable
overexpression	vegetative growth: decreased rate
reduction of function	vegetative growth: decreased rate
unspecified	viable
Large-scale survey
null	inviable resistance to St. John's Wort extract: decreased starvation resistance: decreased
overexpression	cell cycle progression in G1 phase: abnormal
reduction of function	competitive fitness: increased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	61 total interaction(s) for 38 unique genes/features.
Physical Interactions	Affinity Capture-MS: 5 Affinity Capture-RNA: 3 Affinity Capture-Western: 7 Biochemical Activity: 1 Co-localization: 3 PCA: 1 Reconstituted Complex: 3 Two-hybrid: 2
Genetic Interactions	Dosage Rescue: 1 Phenotypic Suppression: 1 Synthetic Growth Defect: 3 Synthetic Lethality: 9 Synthetic Rescue: 22
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIV:1266898 to 1266366 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..37 1266898..1266862 2011-02-03 1996-07-31 Intron 38..129 1266861..1266770 2011-02-03 1996-07-31 CDS 130..533 1266769..1266366 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS) Intron (38 - 129)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000002805