NCB2/YDR397C Summary Help

Standard Name NCB2 1
Systematic Name YDR397C
Alias YDR1
Feature Type ORF, Verified
Description Subunit of a heterodimeric NC2 transcription regulator complex; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta; complex also includes Bur6p (1, 2, 3)
Name Description Negative Cofactor B 1
Chromosomal Location
ChrIV:1266898 to 1266366 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NCB2 GO evidence and references
  View Computational GO annotations for NCB2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 105 genes
Regulators 4 genes
Resources
Classical genetics
conditional
null
overexpression
reduction of function
unspecified
Large-scale survey
null
overexpression
reduction of function
Resources
61 total interaction(s) for 38 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 7
  • Biochemical Activity: 1
  • Co-localization: 3
  • PCA: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 1
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 9
  • Synthetic Rescue: 22

Resources
Expression Summary
histogram
Resources
Length (a.a.) 146
Molecular Weight (Da) 16,650
Isoelectric Point (pI) 4.72
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1266898 to 1266366 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..37 1266898..1266862 2011-02-03 1996-07-31
Intron 38..129 1266861..1266770 2011-02-03 1996-07-31
CDS 130..533 1266769..1266366 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002805
References cited on this page View Complete Literature Guide for NCB2
1) Gadbois EL, et al.  (1997) Functional antagonism between RNA polymerase II holoenzyme and global negative regulator NC2 in vivo. Proc Natl Acad Sci U S A 94(7):3145-50
2) Lemaire M, et al.  (2000) The NC2 repressor is dispensable in yeast mutated for the Sin4p component of the holoenzyme and plays roles similar to Mot1p in vivo. Mol Microbiol 36(1):163-73
3) Cang Y and Prelich G  (2002) Direct stimulation of transcription by negative cofactor 2 (NC2) through TATA-binding protein (TBP). Proc Natl Acad Sci U S A 99(20):12727-32