NBP2/YDR162C Summary Help

Standard Name NBP2 1
Systematic Name YDR162C
Feature Type ORF, Verified
Description Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) (2, 3, 4)
Name Description Nap1 Binding Protein 1
Chromosomal Location
ChrIV:781100 to 780390 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NBP2 GO evidence and references
  View Computational GO annotations for NBP2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
517 total interaction(s) for 292 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 6
  • Co-crystal Structure: 1
  • PCA: 21
  • Protein-peptide: 10
  • Reconstituted Complex: 8
  • Two-hybrid: 25

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Lethality: 5
  • Dosage Rescue: 4
  • Negative Genetic: 274
  • Positive Genetic: 25
  • Synthetic Growth Defect: 72
  • Synthetic Lethality: 47
  • Synthetic Rescue: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 236
Molecular Weight (Da) 26,570
Isoelectric Point (pI) 3.84
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:781100 to 780390 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..711 781100..780390 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002569
References cited on this page View Complete Literature Guide for NBP2
1) Shimizu Y, et al.  (2000) NBP1 (Nap1 binding protein 1), an essential gene for G2/M transition of Saccharomyces cerevisiae, encodes a protein of distinct sub-nuclear localization. Gene 246(1-2):395-404
2) Ohkuni K, et al.  (2003) Yeast Nap1-binding protein Nbp2p is required for mitotic growth at high temperatures and for cell wall integrity. Genetics 165(2):517-29
3) Mapes J and Ota IM  (2004) Nbp2 targets the Ptc1-type 2C Ser/Thr phosphatase to the HOG MAPK pathway. EMBO J 23(2):302-11
4) Stanger K, et al.  (2012) Yeast adaptor protein, Nbp2p, is conserved regulator of fungal Ptc1p phosphatases and is involved in multiple signaling pathways. J Biol Chem 287(26):22133-41