NBP2/YDR162C Summary Help

Standard Name NBP2 1
Systematic Name YDR162C
Feature Type ORF, Verified
Description Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) (2, 3, 4)
Name Description Nap1 Binding Protein 1
Chromosomal Location
ChrIV:781100 to 780390 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NBP2 GO evidence and references
Molecular Function
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
518 total interaction(s) for 293 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 6
  • Co-crystal Structure: 1
  • PCA: 21
  • Protein-peptide: 10
  • Reconstituted Complex: 8
  • Two-hybrid: 25

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Lethality: 5
  • Dosage Rescue: 4
  • Negative Genetic: 274
  • Positive Genetic: 25
  • Synthetic Growth Defect: 72
  • Synthetic Lethality: 47
  • Synthetic Rescue: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 236
Molecular Weight (Da) 26,570
Isoelectric Point (pI) 3.84
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:781100 to 780390 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..711 781100..780390 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002569
References cited on this page View Complete Literature Guide for NBP2
1) Shimizu Y, et al.  (2000) NBP1 (Nap1 binding protein 1), an essential gene for G2/M transition of Saccharomyces cerevisiae, encodes a protein of distinct sub-nuclear localization. Gene 246(1-2):395-404
2) Ohkuni K, et al.  (2003) Yeast Nap1-binding protein Nbp2p is required for mitotic growth at high temperatures and for cell wall integrity. Genetics 165(2):517-29
3) Mapes J and Ota IM  (2004) Nbp2 targets the Ptc1-type 2C Ser/Thr phosphatase to the HOG MAPK pathway. EMBO J 23(2):302-11
4) Stanger K, et al.  (2012) Yeast adaptor protein, Nbp2p, is conserved regulator of fungal Ptc1p phosphatases and is involved in multiple signaling pathways. J Biol Chem 287(26):22133-41