NAP1/YKR048C Summary

NAP1/YKR048C Summary

Standard Name	NAP1 1
Systematic Name	YKR048C
Feature Type	ORF, Verified
Description	Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress (2, 3, 4, 5, 6)
Name Description	Nucleosome Assembly Protein 1

Chromosomal Location	ChrXI:526640 to 525387 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All NAP1 GO evidence and references

	View Computational GO annotations for NAP1
Molecular Function
Manually curated	cyclin binding (IPI) histone binding (IDA)
Biological Process
Manually curated	budding cell bud growth (IGI, IMP) M phase of mitotic cell cycle (IGI, IMP) nucleosome assembly (IDA, ISS) nucleosome disassembly (IDA) positive regulation of transcription elongation from RNA polymerase II promoter (IDA) protein import into nucleus (IMP)
Cellular Component
Manually curated	cytoplasm (IDA) colocalizes_with nuclear nucleosome (IDA) nucleus (IPI)

Mutant phenotypes All NAP1 Phenotype evidence and references

Classical genetics
null	bud morphology: abnormal budding index: abnormal cell shape: abnormal chitin deposition: abnormal cold sensitivity: decreased nuclear import: decreased Clb2p activity: delayed Gin4p distribution: abnormal Hsl1p distribution: abnormal Hsl7p distribution: abnormal resistance to benomyl: increased resistance to acetic acid: increased
overexpression	bud morphology: abnormal
Large-scale survey
null	anaerobic growth: decreased competitive fitness: decreased resistance to sirolimus: decreased resistance to dimethyl sulfoxide: decreased resistance to edelfosine: decreased resistance to benzo[a]pyrene: decreased starvation resistance: decreased vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All NAP1 Interaction evidence and references

	442 total interaction(s) for 258 unique genes/features.
Physical Interactions	Affinity Capture-MS: 150 Affinity Capture-RNA: 3 Affinity Capture-Western: 37 Biochemical Activity: 13 Co-crystal Structure: 1 PCA: 14 Reconstituted Complex: 11 Two-hybrid: 24
Genetic Interactions	Dosage Growth Defect: 2 Negative Genetic: 127 Phenotypic Enhancement: 12 Phenotypic Suppression: 3 Positive Genetic: 16 Synthetic Growth Defect: 18 Synthetic Lethality: 4 Synthetic Rescue: 7
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All NAP1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXI:526640 to 525387 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1254	526640..525387	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001756

References cited on this page View Complete Literature Guide for NAP1

1)	Ishimi Y and Kikuchi A (1991) Identification and molecular cloning of yeast homolog of nucleosome assembly protein I which facilitates nucleosome assembly in vitro. J Biol Chem 266(11):7025-9
2)	Kellogg DR and Murray AW (1995) NAP1 acts with Clb1 to perform mitotic functions and to suppress polar bud growth in budding yeast. J Cell Biol 130(3):675-85
3)	Fujii-Nakata T, et al. (1992) Functional analysis of nucleosome assembly protein, NAP-1. The negatively charged COOH-terminal region is not necessary for the intrinsic assembly activity. J Biol Chem 267(29):20980-6
4)	Mosammaparast N, et al. (2002) A role for nucleosome assembly protein 1 in the nuclear transport of histones H2A and H2B. EMBO J 21(23):6527-38
5)	Calvert ME, et al. (2008) Phosphorylation by casein kinase 2 regulates Nap1 localization and function. Mol Cell Biol 28(4):1313-25
6)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76