NAM2/YLR382C Summary Help

NAM2 BASIC INFORMATION

Standard Name NAM2 1
Systematic Name YLR382C
Alias MSL1
Feature Type ORF, Verified
Description Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance (2, 3, 4, 5 and see Summary Paragraph)
Name Description Nuclear Accommodation of Mitochondria 1
Gene Product Alias LeuRS 6 , mitochondrial leucyl-tRNA synthetase 6
GO Annotations All NAM2 GO evidence and references
    View Computational GO annotations for NAM2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant Phenotype All NAM2 Phenotype details and references
Large-scale survey
null
Interactions NAM2 All interactions details and references
22 total interaction(s) for 20 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Protein-RNA: 2
  • Two-hybrid: 1
Genetic Interactions
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 10
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 1
Sequence Information
ChrXII:884751 to 882067 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 325 cM
Last Update Coordinates: 2004-02-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2685 884751..882067 2004-02-05 1996-07-31
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000004374

NAM2 RESOURCES

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SGD ORF map
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  • Localization Resources
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  • Functional Analysis

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Expression Summary histogram

ADDITIONAL INFORMATION for NAM2

NOMENCLATURE CONFLICT NOTE

NameRelevanceDescription
MSL1Nomenclature conflictMSL1 has been used in the literature to refer to both NAM2/YLR382C (Tzagoloff, A. et al. (1988) J. Biol. Chem. 263:850; PMID:2826465), which encodes a mitochondrial leucyl tRNA synthetase, and MSL1/YIR009W (Tang, J. et al. (1996) Mol. Cell. Biol. 16:2787; PMID:8649387), which encodes the U2 small nuclear ribonucleoprotein particle B" protein.

SUMMARY PARAGRAPH for NAM2

In a process critical for accurate translation of the genetic code, aminoacyl-tRNA synthetases (aka aminoacyl-tRNA ligases) attach amino acids specifically to cognate tRNAs, thereby "charging" the tRNAs. The catalysis is accomplished via a two-step mechanism. First, the synthetase activates the amino acid in an ATP-dependent reaction, producing aminoacyl-adenylate and releasing inorganic pyrophosphate (PPi). Second, the enzyme binds the correct tRNA and transfers the activated amino acid to either the 2' or 3' terminal hydroxyl group of the tRNA, forming the aminoacyl-tRNA and AMP (7, 8 and references therein).

Aminoacyl-tRNA synthetases possess precise substrate specificity and, despite their similarity in function, vary in size, primary sequence and subunit composition. Individual members of the aminoacyl-tRNA synthetase family can be categorized in one of two classes, depending on amino acid specificity. Class I enzymes (those specific for Glu, Gln, Arg, Cys, Met, Val, Ile, Leu, Tyr and Trp) typically contain two highly conserved sequence motifs, are monomeric or dimeric, and aminoacylate at the 2' terminal hydroxyl of the appropriate tRNA. Class II enzymes (those specific for Gly, Ala, Pro, Ser, Thr, His, Asp, Asn, Lys and Phe) typically contain three highly conserved sequence motifs, are dimeric or tetrameric, and aminoacylate at the 3' terminal hydroxyl of the appropriate tRNA (7, 8, 9 and references therein).

Last updated: 2008-07-14

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for NAM2]

1) Dujardin G, et al.  (1980) Long range control circuits within mitochondria and between nucleus and mitochondria. I. Methodology and phenomenology of suppressors. Mol Gen Genet 179(3):469-82
2) Labouesse M  (1990) The yeast mitochondrial leucyl-tRNA synthetase is a splicing factor for the excision of several group I introns. Mol Gen Genet 224(2):209-21
3) Zagorski W, et al.  (1991) Purification and characterization of the Saccharomyces cerevisiae mitochondrial leucyl-tRNA synthetase. J Biol Chem 266(4):2537-41
4) Labouesse M, et al.  (1985) The yeast nuclear gene NAM2 is essential for mitochondrial DNA integrity and can cure a mitochondrial RNA-maturase deficiency. Cell 41(1):133-43
5) Rho SB and Martinis SA  (2000) The bI4 group I intron binds directly to both its protein splicing partners, a tRNA synthetase and maturase, to facilitate RNA splicing activity. RNA 6(12):1882-94
6) Herbert CJ, et al.  (1988) The NAM2 proteins from S. cerevisiae and S. douglasii are mitochondrial leucyl-tRNA synthetases, and are involved in mRNA splicing. EMBO J 7(2):473-83
7) Delarue M  (1995) Aminoacyl-tRNA synthetases. Curr Opin Struct Biol 5(1):48-55
8) Arnez JG and Moras D  (1997) Structural and functional considerations of the aminoacylation reaction. Trends Biochem Sci 22(6):211-6
9) Eriani G, et al.  (1990) Partition of tRNA synthetases into two classes based on mutually exclusive sets of sequence motifs. Nature 347(6289):203-6