NAB3/YPL190C Summary Help

Standard Name NAB3 1
Systematic Name YPL190C
Alias HMD1
Feature Type ORF, Verified
Description RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with exosome to mediate 3'-end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing; Nrd1-Nab3 pathway appears to have a role in rapid suppression of some genes when cells are shifted to poor growth conditions, indicating role for Nrd1-Nab3 in regulating cellular response to nutrient availability (1, 2, 3, 4, 5)
Name Description Nuclear polyAdenylated RNA-Binding 1
Chromosomal Location
ChrXVI:187725 to 185317 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NAB3 GO evidence and references
  View Computational GO annotations for NAB3
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
conditional
null
repressible
Large-scale survey
conditional
null
overexpression
Resources
115 total interaction(s) for 77 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 44
  • Affinity Capture-RNA: 40
  • Affinity Capture-Western: 6
  • Biochemical Activity: 5
  • Co-localization: 1
  • PCA: 3
  • Reconstituted Complex: 2
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Rescue: 3
  • Phenotypic Enhancement: 2
  • Synthetic Growth Defect: 2
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 802
Molecular Weight (Da) 90,438
Isoelectric Point (pI) 4.22
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:187725 to 185317 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2409 187725..185317 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006111
References cited on this page View Complete Literature Guide for NAB3
1) Wilson SM, et al.  (1994) Characterization of nuclear polyadenylated RNA-binding proteins in Saccharomyces cerevisiae. J Cell Biol 127(5):1173-84
2) Conrad NK, et al.  (2000) A yeast heterogeneous nuclear ribonucleoprotein complex associated with RNA polymerase II. Genetics 154(2):557-71
3) Steinmetz EJ, et al.  (2001) RNA-binding protein Nrd1 directs poly(A)-independent 3'-end formation of RNA polymerase II transcripts. Nature 413(6853):327-31
4) Carroll KL, et al.  (2004) Identification of cis elements directing termination of yeast nonpolyadenylated snoRNA transcripts. Mol Cell Biol 24(14):6241-52
5) Darby MM, et al.  (2012) The Saccharomyces cerevisiae Nrd1-Nab3 transcription termination pathway acts in opposition to Ras signaling and mediates response to nutrient depletion. Mol Cell Biol 32(10):1762-75