NAB2/YGL122C Summary Help

Standard Name NAB2 1
Systematic Name YGL122C
Feature Type ORF, Verified
Description Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; involved in forming export-competent mRNPs in the nucleus; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5, 6, 7, 8, 9)
Name Description Nuclear polyAdenylated RNA-Binding 1
Chromosomal Location
ChrVII:280523 to 278946 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NAB2 GO evidence and references
  View Computational GO annotations for NAB2
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
conditional
null
Large-scale survey
null
overexpression
Resources
2695 total interaction(s) for 2582 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 44
  • Affinity Capture-RNA: 2541
  • Affinity Capture-Western: 27
  • Biochemical Activity: 5
  • Co-crystal Structure: 1
  • Co-localization: 1
  • PCA: 2
  • Reconstituted Complex: 14
  • Two-hybrid: 24

Genetic Interactions
  • Dosage Growth Defect: 4
  • Dosage Lethality: 1
  • Dosage Rescue: 7
  • Negative Genetic: 3
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 3
  • Synthetic Rescue: 8

Resources
Expression Summary
histogram
Resources
Length (a.a.) 525
Molecular Weight (Da) 58,321
Isoelectric Point (pI) 8.01
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:280523 to 278946 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1578 280523..278946 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003090
References cited on this page View Complete Literature Guide for NAB2
1) Anderson JT, et al.  (1993) NAB2: a yeast nuclear polyadenylated RNA-binding protein essential for cell viability. Mol Cell Biol 13(5):2730-41
2) Wilson SM, et al.  (1994) Characterization of nuclear polyadenylated RNA-binding proteins in Saccharomyces cerevisiae. J Cell Biol 127(5):1173-84
3) Truant R, et al.  (1998) Identification and functional characterization of a novel nuclear localization signal present in the yeast Nab2 poly(A)+ RNA binding protein. Mol Cell Biol 18(3):1449-58
4) Green DM, et al.  (2002) Nab2p is required for poly(A) RNA export in Saccharomyces cerevisiae and is regulated by arginine methylation via Hmt1p. J Biol Chem 277(10):7752-60
5) Hector RE, et al.  (2002) Dual requirement for yeast hnRNP Nab2p in mRNA poly(A) tail length control and nuclear export. EMBO J 21(7):1800-10
6) Roth KM, et al.  (2005) The nuclear exosome contributes to autogenous control of NAB2 mRNA levels. Mol Cell Biol 25(5):1577-85
7) Fasken MB, et al.  (2008) Functional Significance of the Interaction between the mRNA-binding Protein, Nab2, and the Nuclear Pore-associated Protein, Mlp1, in mRNA Export. J Biol Chem 283(40):27130-43
8) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
9) Brockmann C, et al.  (2012) Structural basis for polyadenosine-RNA binding by Nab2 Zn fingers and its function in mRNA nuclear export. Structure 20(6):1007-18