MYO3/YKL129C Summary

Standard Name	MYO3 1
Systematic Name	YKL129C
Feature Type	ORF, Verified
Description	One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; MYO3 has a paralog, MYO5, that arose from the whole genome duplication (2, 3, 4, 5 and see Summary Paragraph)
Name Description	MYOsin 1

Chromosomal Location	ChrXI:200167 to 196349 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All MYO3 GO evidence and references

	View Computational GO annotations for MYO3
Molecular Function
Manually curated	microfilament motor activity (TAS)
Biological Process
Manually curated	actin cortical patch localization (IGI) bipolar cellular bud site selection (TAS) endocytosis (TAS) exocytosis (TAS) fungal-type cell wall organization (TAS) response to osmotic stress (TAS)
Cellular Component
Manually curated	actin cortical patch (TAS)

Mutant phenotypes All MYO3 Phenotype evidence and references

Classical genetics
null	viable
Large-scale survey
null	budding index: increased competitive fitness: decreased mitochondrial genome maintenance: abnormal resistance to mefloquine: decreased vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All MYO3 Interaction evidence and references

	124 total interaction(s) for 65 unique genes/features.
Physical Interactions	Affinity Capture-MS: 19 Affinity Capture-RNA: 5 Affinity Capture-Western: 12 Biochemical Activity: 3 Protein-peptide: 1 Reconstituted Complex: 3 Two-hybrid: 48
Genetic Interactions	Dosage Rescue: 3 Negative Genetic: 10 Phenotypic Enhancement: 4 Phenotypic Suppression: 2 Positive Genetic: 1 Synthetic Growth Defect: 4 Synthetic Lethality: 8 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All MYO3 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXI:200167 to 196349 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 2011-02-03

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3819	200167..196349	2011-02-03	2011-02-03

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001612

SUMMARY PARAGRAPH for MYO3

MYO3 is a non-essential gene that encodes one of two class I myosins found in Saccharomyces cerevisiae (6). Both Myo3p and the other yeast class I myosin, Myo5p, localize to actin cortical patches (7, 8). Cortical patches are found at sites of polarized cell growth, and appear to be involved in endocytosis and cell wall biogenesis (9, 8). Deletion of either MYO3 or MYO5 has little effect on cell growth, but the myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization (1, , 2). In a myo3 null background, the temperature sensitive myo5-1 mutant has a defect in the internalization step of endocytosis (2). Class I myosins play several different roles in many other eukaryotes; see reference 10 for review.

Last updated: 2000-03-24

References cited on this page View Complete Literature Guide for MYO3

1)	Goodson HV and Spudich JA (1995) Identification and molecular characterization of a yeast myosin I. Cell Motil Cytoskeleton 30(1):73-84
2)	Geli MI and Riezman H (1996) Role of type I myosins in receptor-mediated endocytosis in yeast. Science 272(5261):533-5
3)	Anderson BL, et al. (1998) The Src homology domain 3 (SH3) of a yeast type I myosin, Myo5p, binds to verprolin and is required for targeting to sites of actin polarization. J Cell Biol 141(6):1357-70
4)	Tanaka K and Matsui Y (2001) Functions of unconventional myosins in the yeast Saccharomyces cerevisiae. Cell Struct Funct 26(6):671-5
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6)	Brown SS (1997) Myosins in yeast. Curr Opin Cell Biol 9(1):44-8
7)	Goodson HV, et al. (1996) Synthetic lethality screen identifies a novel yeast myosin I gene (MYO5): myosin I proteins are required for polarization of the actin cytoskeleton. J Cell Biol 133(6):1277-91
8)	Pruyne D and Bretscher A (2000) Polarization of cell growth in yeast. J Cell Sci 113 ( Pt 4):571-85
9)	Botstein D, et al. (1997) "The yeast cytoskeleton." Pp. 1-90 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
10)	Mooseker MS and Cheney RE (1995) Unconventional myosins. Annu Rev Cell Dev Biol 11():633-75