MUD2/YKL074C Summary Help

Standard Name MUD2 1
Systematic Name YKL074C
Feature Type ORF, Verified
Description Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 (1, 2)
Name Description Mutant U1 Die 3
Chromosomal Location
ChrXI:296193 to 294610 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All MUD2 GO evidence and references
  View Computational GO annotations for MUD2
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
231 total interaction(s) for 156 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 38
  • Affinity Capture-Western: 3
  • Co-fractionation: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 25

Genetic Interactions
  • Negative Genetic: 118
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 1
  • Positive Genetic: 22
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 10
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 527
Molecular Weight (Da) 60,459
Isoelectric Point (pI) 8.23
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:296193 to 294610 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1584 296193..294610 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001557
References cited on this page View Complete Literature Guide for MUD2
1) Abovich N, et al.  (1994) The yeast MUD2 protein: an interaction with PRP11 defines a bridge between commitment complexes and U2 snRNP addition. Genes Dev 8(7):843-54
2) Rutz B and Seraphin B  (1999) Transient interaction of BBP/ScSF1 and Mud2 with the splicing machinery affects the kinetics of spliceosome assembly. RNA 5(6):819-31
3) Liao XC, et al.  (1993) An enhancer screen identifies a gene that encodes the yeast U1 snRNP A protein: implications for snRNP protein function in pre-mRNA splicing. Genes Dev 7(3):419-28