FLO11/YIR019C Summary

FLO11/YIR019C Summary

Standard Name	FLO11 1
Systematic Name	YIR019C
Alias	STA4 , MUC1 2
Feature Type	ORF, Verified
Description	GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain; a portion is cleaved and shed from cells, and free extracellular Flo11p contributes to the surface properties of cells (2, 3, 4, 5, 6, 7)
Name Description	FLOcculation

Chromosomal Location	ChrIX:393675 to 389572 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All FLO11 GO evidence and references

	View Computational GO annotations for FLO11
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	biofilm formation (IMP) cell-cell adhesion (IMP) filamentous growth (IDA) flocculation (IMP) invasive growth in response to glucose limitation (IMP) pseudohyphal growth (IMP)
Cellular Component
Manually curated	cellular bud neck (IDA) extracellular region (IDA) plasma membrane (IDA)

Mutant phenotypes All FLO11 Phenotype evidence and references

Classical genetics
null	biofilm formation: absent colony appearance: abnormal colony shape: abnormal filamentous growth: decreased flocculation: absent invasive growth: absent pseudohyphal growth: absent
overexpression	adhesion: increased biofilm formation: increased flocculation: increased invasive growth: increased invasive growth: absent
unspecified	pseudohyphal growth: absent
Large-scale survey
null	competitive fitness: increased resistance to cycloheximide: decreased resistance to amitrole: decreased vegetative growth: increased rate viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All FLO11 Interaction evidence and references

	86 total interaction(s) for 78 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-RNA: 1 Reconstituted Complex: 2
Genetic Interactions	Dosage Rescue: 2 Negative Genetic: 22 Phenotypic Enhancement: 4 Phenotypic Suppression: 51 Positive Genetic: 2 Synthetic Lethality: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All FLO11 Protein evidence and references

Localization	YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIX:393675 to 389572 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1994-12-10

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..4104	393675..389572	2011-02-03	1994-12-10

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001458

References cited on this page View Complete Literature Guide for FLO11

1)	Lo WS and Dranginis AM (1996) FLO11, a yeast gene related to the STA genes, encodes a novel cell surface flocculin. J Bacteriol 178(24):7144-51
2)	Lambrechts MG, et al. (1996) Muc1, a mucin-like protein that is regulated by Mss10, is critical for pseudohyphal differentiation in yeast. Proc Natl Acad Sci U S A 93(16):8419-24
3)	Rupp S, et al. (1999) MAP kinase and cAMP filamentation signaling pathways converge on the unusually large promoter of the yeast FLO11 gene. EMBO J 18(5):1257-69
4)	Guo B, et al. (2000) A Saccharomyces gene family involved in invasive growth, cell-cell adhesion, and mating. Proc Natl Acad Sci U S A 97(22):12158-63
5)	Douglas LM, et al. (2007) Expression and Characterization of the Flocculin Flo11/Muc1, a Saccharomyces cerevisiae Mannoprotein with Homotypic Properties of Adhesion. Eukaryot Cell 6(12):2214-21
6)	Karunanithi S, et al. (2010) Shedding of the Mucin-Like Flocculin Flo11p Reveals a New Aspect of Fungal Adhesion Regulation. Curr Biol 20(15):1389-1395
7)	Vachova L, et al. (2011) Flo11p, drug efflux pumps, and the extracellular matrix cooperate to form biofilm yeast colonies. J Cell Biol 194(5):679-87