MTF1/YMR228W Summary Help

Standard Name MTF1 1
Systematic Name YMR228W
Feature Type ORF, Verified
Description Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation (2, 3, 4, 5, 6)
Name Description Mitochondrial Transcription Factor 1
Chromosomal Location
ChrXIII:724626 to 725651 | ORF Map | GBrowse
Gbrowse
Genetic position: 157.9 cM
Gene Ontology Annotations All MTF1 GO evidence and references
  View Computational GO annotations for MTF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 60 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
31 total interaction(s) for 22 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Biochemical Activity: 1
  • Co-purification: 1
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Rescue: 2
  • Phenotypic Enhancement: 1
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 4
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 341
Molecular Weight (Da) 39,726
Isoelectric Point (pI) 6.52
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:724626 to 725651 | ORF Map | GBrowse
SGD ORF map
Genetic position: 157.9 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1026 724626..725651 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004841
References cited on this page View Complete Literature Guide for MTF1
1) Lisowsky T and Michaelis G  (1989) Mutations in the genes for mitochondrial RNA polymerase and a second mitochondrial transcription factor of Saccharomyces cerevisiae. Mol Gen Genet 219(1-2):125-8
2) Mangus DA, et al.  (1994) Release of the yeast mitochondrial RNA polymerase specificity factor from transcription complexes. J Biol Chem 269(42):26568-74
3) Schubot FD, et al.  (2001) Crystal structure of the transcription factor sc-mtTFB offers insights into mitochondrial transcription. Protein Sci 10(10):1980-8
4) Matsunaga M and Jaehning JA  (2004) Intrinsic promoter recognition by a "core" RNA polymerase. J Biol Chem 279(43):44239-42
5) Tang GQ, et al.  (2011) Transcription factor-dependent DNA bending governs promoter recognition by the mitochondrial RNA polymerase. J Biol Chem 286(44):38805-13
6) Velazquez G, et al.  (2012) Conservation of promoter melting mechanisms in divergent regions of the single-subunit RNA polymerases. Biochemistry 51(18):3901-10