MSL5/YLR116W Summary Help

Standard Name MSL5 1
Systematic Name YLR116W
Feature Type ORF, Verified
Description Component of commitment complex; which defines first step in splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA; relocalizes to the cytosol in response to hypoxia (1, 2, 3)
Name Description Mud Synthetic-Lethal 1
Gene Product Alias BBP 1
Chromosomal Location
ChrXII:380822 to 382252 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MSL5 GO evidence and references
  View Computational GO annotations for MSL5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
conditional
null
reduction of function
Large-scale survey
conditional
null
overexpression
reduction of function
unspecified
Resources
88 total interaction(s) for 67 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 43
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 4
  • Co-fractionation: 1
  • Protein-RNA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 18

Genetic Interactions
  • Negative Genetic: 6
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 3
  • Synthetic Lethality: 6
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 476
Molecular Weight (Da) 53,034
Isoelectric Point (pI) 10.49
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:380822 to 382252 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1431 380822..382252 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004106
References cited on this page View Complete Literature Guide for MSL5
1) Abovich N and Rosbash M  (1997) Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals. Cell 89(3):403-12
2) Rutz B and Seraphin B  (2000) A dual role for BBP/ScSF1 in nuclear pre-mRNA retention and splicing. EMBO J 19(8):1873-86
3) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30