Standard Name	MSL5 1
Systematic Name	YLR116W
Feature Type	ORF, Verified
Description	Component of commitment complex; which defines first step in splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA; relocalizes to the cytosol in response to hypoxia (1, 2, 3)
Name Description	Mud Synthetic-Lethal 1
Gene Product Alias	BBP 1

Chromosomal Location	ChrXII:380822 to 382252 | ORF Map | GBrowse

	View Computational GO annotations for MSL5
Molecular Function
Manually curated	pre-mRNA branch point binding (IDA)
Biological Process
Manually curated	mRNA splicing, via spliceosome (IPI)
Cellular Component
Manually curated	commitment complex (IDA) cytosol (IDA) nucleus (IDA)

Classical genetics
conditional	colony sectoring: increased
null	inviable
reduction of function	unspliced pre-mRNA accumulation: increased
Large-scale survey
conditional	heat sensitivity: increased
null	inviable
overexpression	vegetative growth: decreased vegetative growth: decreased rate
reduction of function	competitive fitness: decreased resistance to methyl methanesulfonate: decreased
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	87 total interaction(s) for 66 unique genes/features.
Physical Interactions	Affinity Capture-MS: 43 Affinity Capture-RNA: 1 Affinity Capture-Western: 4 Co-fractionation: 1 Protein-RNA: 1 Reconstituted Complex: 1 Two-hybrid: 18
Genetic Interactions	Negative Genetic: 6 Phenotypic Enhancement: 1 Phenotypic Suppression: 1 Positive Genetic: 3 Synthetic Lethality: 6 Synthetic Rescue: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXII:380822 to 382252 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1431 380822..382252 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000004106