Standard Name	MSB4 1
Systematic Name	YOL112W
Feature Type	ORF, Verified
Description	GTPase-activating protein of the Ras superfamily; acts primarily on Sec4p, localizes to the bud site and bud tip; msb3 msb4 double mutation causes defects in secretion and actin organization; MSB4 has a paralog, MSB3, that arose from the whole genome duplication (2, 3, 4, 5)
Name Description	Multicopy Suppression of a Budding defect 1, 6

Chromosomal Location	ChrXV:106710 to 108188 | ORF Map | GBrowse

	View Computational GO annotations for MSB4
Molecular Function
Manually curated	Rab GTPase activator activity (IDA)
Biological Process
Manually curated	actin filament organization (IGI, IPI) exocytosis (IGI)
Cellular Component
Manually curated	cellular bud tip (IDA) incipient cellular bud site (IDA) polarisome (IPI)

Classical genetics
overexpression	actin cytoskeleton morphology: abnormal cell cycle progression in G2 phase: abnormal
Large-scale survey
null	competitive fitness: increased viable
overexpression	vegetative growth: decreased vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	99 total interaction(s) for 91 unique genes/features.
Physical Interactions	Affinity Capture-RNA: 2 Biochemical Activity: 4 PCA: 1 Reconstituted Complex: 5 Two-hybrid: 1
Genetic Interactions	Dosage Growth Defect: 2 Dosage Lethality: 3 Dosage Rescue: 9 Negative Genetic: 57 Phenotypic Enhancement: 2 Synthetic Growth Defect: 6 Synthetic Lethality: 7
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXV:106710 to 108188 | ORF Map | GBrowse
Last Update	Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1479 106710..108188 2006-01-05 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000005472