MSB2/YGR014W Summary Help

Standard Name MSB2 1
Systematic Name YGR014W
Feature Type ORF, Verified
Description Mucin family member involved in various signaling pathways; functions as osmosensor in Sho1p-mediated HOG pathway with Msb2p; functions in Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; processed into secreted and cell-associated forms by aspartyl protease Yps1p; potential Cdc28p substrate (2, 3, 4, 5, 6, 7, 8)
Name Description Multicopy Suppressor of a Budding defect
Chromosomal Location
ChrVII:516943 to 520863 | ORF Map | GBrowse
Genetic position: 2 cM
Gene Ontology Annotations All MSB2 GO evidence and references
  View Computational GO annotations for MSB2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
Large-scale survey
70 total interaction(s) for 53 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 6
  • Biochemical Activity: 1
  • PCA: 4
  • Reconstituted Complex: 1
  • Two-hybrid: 10

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 5
  • Negative Genetic: 14
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 8
  • Positive Genetic: 1
  • Synthetic Growth Defect: 2
  • Synthetic Haploinsufficiency: 2
  • Synthetic Lethality: 3
  • Synthetic Rescue: 6

Expression Summary
Length (a.a.) 1,306
Molecular Weight (Da) 133,113
Isoelectric Point (pI) 3.9
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:516943 to 520863 | ORF Map | GBrowse
Genetic position: 2 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3921 516943..520863 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003246
References cited on this page View Complete Literature Guide for MSB2
1) Bender, A. and Pringle, J.  (1992) Personal Communication, Mortimer Map Edition 11
2) Bender A and Pringle JR  (1989) Multicopy suppression of the cdc24 budding defect in yeast by CDC42 and three newly identified genes including the ras-related gene RSR1. Proc Natl Acad Sci U S A 86(24):9976-80
3) O'Rourke SM and Herskowitz I  (2002) A third osmosensing branch in Saccharomyces cerevisiae requires the Msb2 protein and functions in parallel with the Sho1 branch. Mol Cell Biol 22(13):4739-49
4) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
5) Cullen PJ, et al.  (2004) A signaling mucin at the head of the Cdc42- and MAPK-dependent filamentous growth pathway in yeast. Genes Dev 18(14):1695-708
6) Vadaie N, et al.  (2008) Cleavage of the signaling mucin Msb2 by the aspartyl protease Yps1 is required for MAPK activation in yeast. J Cell Biol 181(7):1073-81
7) Hohmann S  (2009) Control of high osmolarity signalling in the yeast Saccharomyces cerevisiae. FEBS Lett 583(24):4025-9
8) Rodriguez-Pena JM, et al.  (2010) The high-osmolarity glycerol (HOG) and cell wall integrity (CWI) signalling pathways interplay: a yeast dialogue between MAPK routes. Yeast 27(8):495-502