MSB1/YOR188W Summary Help

Standard Name MSB1
Systematic Name YOR188W
Feature Type ORF, Verified
Description Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress (1, 2, 3, 4 and see Summary Paragraph)
Name Description Multicopy Suppressor of a Budding defect
Chromosomal Location
ChrXV:685767 to 689180 | ORF Map | GBrowse
Genetic position: 103 cM
Gene Ontology Annotations All MSB1 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Classical genetics
Large-scale survey
57 total interaction(s) for 32 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 7
  • Biochemical Activity: 1
  • PCA: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 4
  • Dosage Lethality: 1
  • Dosage Rescue: 18
  • Negative Genetic: 5
  • Phenotypic Enhancement: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 3
  • Synthetic Rescue: 3

Expression Summary
Length (a.a.) 1,137
Molecular Weight (Da) 129,970
Isoelectric Point (pI) 9.55
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXV:685767 to 689180 | ORF Map | GBrowse
Genetic position: 103 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3414 685767..689180 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005714

MSB1 was first identified as a high-copy suppressor of a temperature sensitive cdc24 mutation, and was also found to suppress mutations in cdc42 (1). Because of these genetic interactions with genes involved in the establishment of cell polarity and bud formation, Msb1p is thought to play a role in cell morphogenesis. Cells mutant for the genes BEM1 and BEM2, thought to be involved in bud emergence, require MSB1 for viability (5). A deletion of MSB1 does not produce a readily detectable phenotype (5).

Last updated: 1999-09-01 Contact SGD

References cited on this page View Complete Literature Guide for MSB1
1) Bender A and Pringle JR  (1989) Multicopy suppression of the cdc24 budding defect in yeast by CDC42 and three newly identified genes including the ras-related gene RSR1. Proc Natl Acad Sci U S A 86(24):9976-80
2) Sekiya-Kawasaki M, et al.  (2002) Dissection of upstream regulatory components of the Rho1p effector, 1,3-beta-glucan synthase, in Saccharomyces cerevisiae. Genetics 162(2):663-76
3) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5) Bender A and Pringle JR  (1991) Use of a screen for synthetic lethal and multicopy suppressee mutants to identify two new genes involved in morphogenesis in Saccharomyces cerevisiae. Mol Cell Biol 11(3):1295-305