MRH4/YGL064C Summary Help

Standard Name MRH4 1
Systematic Name YGL064C
Feature Type ORF, Verified
Description Mitochondrial ATP-dependent RNA helicase of the DEAD-box family; required for assembly of the large subunit of mitochondrial ribosomes; binds to the large subunit rRNA, 21S_rRNA; localizes to the matrix face of the mitochondrial inner membrane and associates with the large subunit precursor and with mature ribosomes (1, 2)
Name Description Mitochondrial RNA Helicase 1
Chromosomal Location
ChrVII:383208 to 381523 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All MRH4 GO evidence and references
  View Computational GO annotations for MRH4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 7 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
41 total interaction(s) for 38 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Co-fractionation: 1
  • PCA: 1

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 12
  • Positive Genetic: 12
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 561
Molecular Weight (Da) 63,057
Isoelectric Point (pI) 10.75
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:383208 to 381523 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1686 383208..381523 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003032
References cited on this page View Complete Literature Guide for MRH4
1) Schmidt U, et al.  (2002) A novel mitochondrial DEAD box protein (Mrh4) required for maintenance of mtDNA in Saccharomyces cerevisiae. FEMS Yeast Res 2(3):267-76
2) De Silva D, et al.  (2013) The DEAD Box Protein Mrh4 Functions in the Assembly of the Mitochondrial Large Ribosomal Subunit. Cell Metab 18(5):712-25