MPT5/YGL178W Summary Help

Standard Name MPT5 1
Systematic Name YGL178W
Alias HTR1 , PUF5 , UTH4
Feature Type ORF, Verified
Description mRNA-binding protein of the PUF family; binds to the 3' UTR of specific mRNAs, including those involved in mating type switching, cell wall integrity, chronological lifespan, chromatin modification, and spindle pole body architecture; recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity (1, 2, 3, 4, 5, 6, 7, 8, 9)
Name Description Multicopy suppressor of Pop Two 10
Chromosomal Location
ChrVII:167352 to 170571 | ORF Map | GBrowse
Gene Ontology Annotations All MPT5 GO evidence and references
  View Computational GO annotations for MPT5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
Large-scale survey
84 total interaction(s) for 52 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 8
  • PCA: 10
  • Protein-RNA: 3
  • Two-hybrid: 10

Genetic Interactions
  • Dosage Rescue: 12
  • Negative Genetic: 8
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 1
  • Positive Genetic: 3
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 6
  • Synthetic Rescue: 5

Expression Summary
Length (a.a.) 859
Molecular Weight (Da) 95,421
Isoelectric Point (pI) 8.02
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:167352 to 170571 | ORF Map | GBrowse
This feature contains embedded feature(s): YGL177W
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3 167352..167354 2011-02-03 1996-07-31
Intron 4..643 167355..167994 2011-02-03 1996-07-31
CDS 644..3220 167995..170571 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003146
References cited on this page View Complete Literature Guide for MPT5
1) Chen T and Kurjan J  (1997) Saccharomyces cerevisiae Mpt5p interacts with Sst2p and plays roles in pheromone sensitivity and recovery from pheromone arrest. Mol Cell Biol 17(6):3429-39
2) Kennedy BK, et al.  (1997) Redistribution of silencing proteins from telomeres to the nucleolus is associated with extension of life span in S. cerevisiae. Cell 89(3):381-91
3) Tadauchi T, et al.  (2001) Post-transcriptional regulation through the HO 3'-UTR by Mpt5, a yeast homolog of Pumilio and FBF. EMBO J 20(3):552-61
4) Kaeberlein M and Guarente L  (2002) Saccharomyces cerevisiae MPT5 and SSD1 function in parallel pathways to promote cell wall integrity. Genetics 160(1):83-95
5) Gerber AP, et al.  (2004) Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast. PLoS Biol 2(3):E79
6) Goldstrohm AC, et al.  (2006) PUF proteins bind Pop2p to regulate messenger RNAs. Nat Struct Mol Biol 13(6):533-9
7) Stewart MS, et al.  (2007) Mpt5p, a stress tolerance- and lifespan-promoting PUF protein in Saccharomyces cerevisiae, acts upstream of the cell wall integrity pathway. Eukaryot Cell 6(2):262-70
8) Traven A, et al.  (2010) The yeast PUF protein Puf5 has Pop2-independent roles in response to DNA replication stress. PLoS One 5(5):e10651
9) Cridge AG, et al.  (2010) Identifying eIF4E-binding protein translationally-controlled transcripts reveals links to mRNAs bound by specific PUF proteins. Nucleic Acids Res 38(22):8039-50
10) Hata H, et al.  (1998) Dhh1p, a putative RNA helicase, associates with the general transcription factors Pop2p and Ccr4p from Saccharomyces cerevisiae. Genetics 148(2):571-9