MPS3/YJL019W Summary Help

Standard Name MPS3 1
Systematic Name YJL019W
Alias NEP98 2 , YJL018W
Feature Type ORF, Verified
Description Nuclear envelope protein; required for SPB insertion, initiation of SPB duplication and nuclear fusion; interacts with Mps2p to tether half-bridge to core SPB; N-terminal acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; member of the SUN protein family (Sad1-UNC-84 homology) (1, 2, 3, 4, 5, 6, 7)
Name Description MonoPolar Spindle 1
Chromosomal Location
ChrX:402897 to 404945 | ORF Map | GBrowse
Gene Ontology Annotations All MPS3 GO evidence and references
  View Computational GO annotations for MPS3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
reduction of function
Large-scale survey
229 total interaction(s) for 174 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 9
  • Biochemical Activity: 1
  • Co-localization: 3
  • Far Western: 1
  • Protein-RNA: 1
  • Reconstituted Complex: 11
  • Two-hybrid: 58

Genetic Interactions
  • Dosage Rescue: 6
  • Negative Genetic: 2
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 3
  • Positive Genetic: 3
  • Synthetic Growth Defect: 60
  • Synthetic Lethality: 19
  • Synthetic Rescue: 44

Expression Summary
Length (a.a.) 682
Molecular Weight (Da) 79,174
Isoelectric Point (pI) 5
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrX:402897 to 404945 | ORF Map | GBrowse
This feature contains embedded feature(s): YJL018W
Last Update Coordinates: 2011-02-03 | Sequence: 2003-01-02
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2049 402897..404945 2011-02-03 2003-01-02
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003556
References cited on this page View Complete Literature Guide for MPS3
1) Jaspersen SL, et al.  (2002) Mps3p is a novel component of the yeast spindle pole body that interacts with the yeast centrin homologue Cdc31p. J Cell Biol 159(6):945-56
2) Nishikawa S, et al.  (2003) Nep98p is a component of the yeast spindle pole body and essential for nuclear division and fusion. J Biol Chem 278(11):9938-43
3) Jaspersen SL, et al.  (2006) The Sad1-UNC-84 homology domain in Mps3 interacts with Mps2 to connect the spindle pole body with the nuclear envelope. J Cell Biol 174(5):665-75
4) Conrad MN, et al.  (2008) Rapid telomere movement in meiotic prophase is promoted by NDJ1, MPS3, and CSM4 and is modulated by recombination. Cell 133(7):1175-87
5) Friederichs JM, et al.  (2011) The SUN Protein Mps3 Is Required for Spindle Pole Body Insertion into the Nuclear Membrane and Nuclear Envelope Homeostasis. PLoS Genet 7(11):e1002365
6) Ghosh S, et al.  (2012) Acetylation of the SUN protein Mps3 by Eco1 regulates its function in nuclear organization. Mol Biol Cell 23(13):2546-59
7) Friederichs JM, et al.  (2012) Genetic Analysis of Mps3 SUN Domain Mutants in Saccharomyces cerevisiae Reveals an Interaction with the SUN-Like Protein Slp1. G3 (Bethesda) 2(12):1703-18