Standard Name	MPE1 1
Systematic Name	YKL059C
Feature Type	ORF, Verified
Description	Essential conserved subunit of CPF cleavage and polyadenylation factor; plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif; relocalizes to the cytosol in response to hypoxia (1, 2)
Name Description	Mutant PCF11 Extragenic suppressor 1

Chromosomal Location	ChrXI:329443 to 328118 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for MPE1
Molecular Function
Manually curated	contributes_to RNA binding (IC)
Biological Process
Manually curated	mRNA cleavage (IDA, IGI, IMP, IPI) mRNA polyadenylation (IDA, IGI, IMP, IPI)
Cellular Component
Manually curated	cytosol (IDA) mRNA cleavage and polyadenylation specificity factor complex (IDA, IPI) nucleus (IDA)

Classical genetics
conditional	heat sensitivity: increased
null	inviable
Large-scale survey
null	haploinsufficient inviable resistance to ethanol: decreased starvation resistance: decreased
overexpression	resistance to sirolimus: decreased vegetative growth: decreased vegetative growth: decreased rate
reduction of function	competitive fitness: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	142 total interaction(s) for 56 unique genes/features.
Physical Interactions	Affinity Capture-MS: 105 Affinity Capture-RNA: 2
Genetic Interactions	Negative Genetic: 28 Positive Genetic: 3 Synthetic Lethality: 2 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXI:329443 to 328118 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
	This feature is contained within: 71006
	This feature is contained within: 70100
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1326 329443..328118 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001542