MOT2/YER068W Summary Help

Standard Name MOT2 1
Systematic Name YER068W
Alias NOT4 , SIG1
Feature Type ORF, Verified
Description Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications; regulates levels of DNA Polymerase-{alpha} to promote efficient and accurate DNA replication (2, 3, 4, 5, 6, 7, 8)
Name Description Modulator Of Transcription 1
Chromosomal Location
ChrV:293050 to 294813 | ORF Map | GBrowse
Gene Ontology Annotations All MOT2 GO evidence and references
  View Computational GO annotations for MOT2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 9 genes
Classical genetics
reduction of function
Large-scale survey
268 total interaction(s) for 166 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 30
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 35
  • Biochemical Activity: 3
  • Co-fractionation: 1
  • Co-purification: 1
  • PCA: 14
  • Reconstituted Complex: 12
  • Two-hybrid: 13

Genetic Interactions
  • Dosage Growth Defect: 6
  • Dosage Lethality: 1
  • Dosage Rescue: 6
  • Negative Genetic: 32
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 4
  • Positive Genetic: 15
  • Synthetic Growth Defect: 38
  • Synthetic Lethality: 48
  • Synthetic Rescue: 5

Expression Summary
Length (a.a.) 587
Molecular Weight (Da) 65,354
Isoelectric Point (pI) 8.04
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrV:293050 to 294813 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1764 293050..294813 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000870
References cited on this page View Complete Literature Guide for MOT2
1) Cade RM and Errede B  (1994) MOT2 encodes a negative regulator of gene expression that affects basal expression of pheromone-responsive genes in Saccharomyces cerevisiae. Mol Cell Biol 14(5):3139-49
2) Bai Y, et al.  (1999) The CCR4 and CAF1 proteins of the CCR4-NOT complex are physically and functionally separated from NOT2, NOT4, and NOT5. Mol Cell Biol 19(10):6642-51
3) Liu HY, et al.  (1998) The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively. EMBO J 17(4):1096-106
4) Badarinarayana V, et al.  (2000) Functional interaction of CCR4-NOT proteins with TATAA-binding protein (TBP) and its associated factors in yeast. Genetics 155(3):1045-54
5) Denis CL, et al.  (2001) Genetic evidence supports a role for the yeast CCR4-NOT complex in transcriptional elongation. Genetics 158(2):627-34
6) Panasenko O, et al.  (2006) The yeast Ccr4-Not complex controls ubiquitination of the nascent-associated polypeptide (NAC-EGD) complex. J Biol Chem 281(42):31389-98
7) Mersman DP, et al.  (2009) Polyubiquitination of the demethylase Jhd2 controls histone methylation and gene expression. Genes Dev 23(8):951-62
8) Haworth J, et al.  (2010) Ubc4 and Not4 Regulate Steady-State Levels of DNA Polymerase-{alpha} to Promote Efficient and Accurate DNA Replication. Mol Biol Cell 21(18):3205-19
9) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104
10) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87