Standard Name	MMS21 1
Systematic Name	YEL019C
Alias	NSE2 2 , PSO10 3
Feature Type	ORF, Verified
Description	SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination (1, 4, 5, 6)
Name Description	Methyl MethaneSulfonate sensitivity 1

Chromosomal Location	ChrV:121301 to 120498 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for MMS21
Molecular Function
Manually curated	SUMO ligase activity (IDA, IMP)
Biological Process
Manually curated	DNA repair (IMP)
Cellular Component
Manually curated	nuclear envelope (IDA) Smc5-Smc6 complex (IDA)
High-throughput	cytoplasm (IDA) nucleus (IDA)

Classical genetics
conditional	UV resistance: decreased cellular morphology: abnormal heat sensitivity: increased resistance to methyl methanesulfonate: decreased
reduction of function	UV resistance: decreased X ray resistance: decreased mutation frequency: increased resistance to methyl methanesulfonate: decreased resistance to hydroxyurea: decreased
unspecified	chromosome segregation: abnormal Matalpha1p accumulation: increased
Large-scale survey
null	inviable resistance to doxorubicin: decreased starvation resistance: decreased
overexpression	resistance to sirolimus: increased
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	75 total interaction(s) for 36 unique genes/features.
Physical Interactions	Affinity Capture-MS: 7 Affinity Capture-Western: 2 Biochemical Activity: 3 Co-crystal Structure: 1 Co-fractionation: 1 Co-purification: 1 Reconstituted Complex: 2 Two-hybrid: 3
Genetic Interactions	Dosage Growth Defect: 1 Dosage Rescue: 1 Negative Genetic: 1 Phenotypic Enhancement: 5 Phenotypic Suppression: 2 Synthetic Growth Defect: 22 Synthetic Lethality: 17 Synthetic Rescue: 6
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Mass Spec

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrV:121301 to 120498 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..804 121301..120498 1996-07-31 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000745