MMS2/YGL087C Summary Help

Standard Name MMS2 1
Systematic Name YGL087C
Feature Type ORF, Verified
Description Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5)
Name Description Methyl MethaneSulfonate sensitivity
Chromosomal Location
ChrVII:346904 to 346406 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All MMS2 GO evidence and references
  View Computational GO annotations for MMS2
Molecular Function
Manually curated
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
unspecified
Resources
192 total interaction(s) for 120 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 1
  • Co-crystal Structure: 2
  • Co-purification: 3
  • PCA: 5
  • Reconstituted Complex: 6
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 61
  • Phenotypic Enhancement: 18
  • Phenotypic Suppression: 7
  • Positive Genetic: 8
  • Synthetic Growth Defect: 34
  • Synthetic Lethality: 12
  • Synthetic Rescue: 17

Resources
Expression Summary
histogram
Resources
Length (a.a.) 137
Molecular Weight (Da) 15,545
Isoelectric Point (pI) 6.76
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:346904 to 346406 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..11 346904..346894 2011-02-03 1996-07-31
Intron 12..96 346893..346809 2011-02-03 1996-07-31
CDS 97..499 346808..346406 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003055
References cited on this page View Complete Literature Guide for MMS2
1) Broomfield S, et al.  (1998) MMS2, encoding a ubiquitin-conjugating-enzyme-like protein, is a member of the yeast error-free postreplication repair pathway. Proc Natl Acad Sci U S A 95(10):5678-83
2) Ulrich HD and Jentsch S  (2000) Two RING finger proteins mediate cooperation between ubiquitin-conjugating enzymes in DNA repair. EMBO J 19(13):3388-97
3) Hofmann RM and Pickart CM  (1999) Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair. Cell 96(5):645-53
4) Ulrich HD  (2003) Protein-protein interactions within an E2-RING finger complex. Implications for ubiquitin-dependent DNA damage repair. J Biol Chem 278(9):7051-8
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76