MMS1/YPR164W Summary Help

Standard Name MMS1 1
Systematic Name YPR164W
Alias RTT108 2 , SLM6
Feature Type ORF, Verified
Description Subunit of E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay (2, 3, 4, 5, 6, 7, 8)
Name Description Methyl MethaneSulfonate sensitivity
Chromosomal Location
ChrXVI:870703 to 874926 | ORF Map | GBrowse
Gene Ontology Annotations All MMS1 GO evidence and references
  View Computational GO annotations for MMS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Classical genetics
reduction of function
Large-scale survey
235 total interaction(s) for 146 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-Western: 10
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Negative Genetic: 99
  • Phenotypic Enhancement: 7
  • Phenotypic Suppression: 5
  • Positive Genetic: 16
  • Synthetic Growth Defect: 69
  • Synthetic Lethality: 10
  • Synthetic Rescue: 4

Expression Summary
Length (a.a.) 1,407
Molecular Weight (Da) 161,236
Isoelectric Point (pI) 5.79
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:870703 to 874926 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..4224 870703..874926 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006368
References cited on this page View Complete Literature Guide for MMS1
1) Prakash L and Prakash S  (1977) Isolation and characterization of MMS-sensitive mutants of Saccharomyces cerevisiae. Genetics 86(1):33-55
2) Scholes DT, et al.  (2001) Multiple regulators of Ty1 transposition in Saccharomyces cerevisiae have conserved roles in genome maintenance. Genetics 159(4):1449-65
3) Hryciw T, et al.  (2002) MMS1 protects against replication-dependent DNA damage in Saccharomyces cerevisiae. Mol Genet Genomics 266(5):848-57
4) Araki Y, et al.  (2003) Budding yeast mcm10/dna43 mutant requires a novel repair pathway for viability. Genes Cells 8(5):465-80
5) Duro E, et al.  (2008) Budding yeast Mms22 and Mms1 regulate homologous recombination induced by replisome blockage. DNA Repair (Amst) 7(5):811-8
6) Zaidi IW, et al.  (2008) Rtt101 and Mms1 in budding yeast form a CUL4(DDB1)-like ubiquitin ligase that promotes replication through damaged DNA. EMBO Rep 9(10):1034-40
7) Fujii K, et al.  (2009) A role for ubiquitin in the clearance of nonfunctional rRNAs. Genes Dev 23(8):963-74
8) Vaisica JA, et al.  (2011) Mms1 and Mms22 stabilize the replisome during replication stress. Mol Biol Cell 22(13):2396-408