MKS1/YNL076W Summary

MKS1/YNL076W Summary

Standard Name	MKS1 1
Systematic Name	YNL076W
Alias	LYS80
Feature Type	ORF, Verified
Description	Pleiotropic negative transcriptional regulator involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling (1, 2, 3, 4)
Name Description	Multicopy Kinase Suppressor 1

Chromosomal Location	ChrXIV:483556 to 485310 \| ORF Map \| GBrowse

Gene Ontology Annotations All MKS1 GO evidence and references

	View Computational GO annotations for MKS1
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	mitochondria-nucleus signaling pathway (IPI) negative regulation of transcription from RNA polymerase II promoter (IGI, IMP) regulation of nitrogen utilization (IMP) regulation of pseudohyphal growth (IMP)
Cellular Component
Manually curated	cytoplasm (IDA)

Mutant phenotypes All MKS1 Phenotype evidence and references

Classical genetics
null	3-mercaptohexan-1-ol accumulation: decreased 3-mercaptohexyl acetate accumulation: decreased Rtg3p modification: abnormal pseudohyphal growth: decreased resistance to sirolimus: increased resistance to sirolimus: decreased resistance to tellurite: increased
unspecified	killer toxin resistance: decreased resistance to caffeine: increased
Large-scale survey
null	competitive fitness: decreased filamentous growth: decreased invasive growth: absent resistance to mycophenolic acid: increased resistance to hydroxyurea: decreased resistance to ethanol: decreased resistance to caffeine: decreased resistance to methyl methanesulfonate: decreased resistance to wortmannin: normal resistance to sirolimus: increased resistance to calcium dichloride: decreased resistance to 5-fluorouracil: decreased stress resistance: decreased toxin resistance: decreased vacuolar morphology: abnormal viable
overexpression	cell cycle progression: abnormal filamentous growth: decreased invasive growth: absent vegetative growth: decreased vegetative growth: decreased rate
unspecified	resistance to Calcofluor White: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All MKS1 Interaction evidence and references

	169 total interaction(s) for 144 unique genes/features.
Physical Interactions	Affinity Capture-MS: 19 Affinity Capture-RNA: 1 Affinity Capture-Western: 10 Reconstituted Complex: 2
Genetic Interactions	Dosage Growth Defect: 1 Dosage Lethality: 1 Negative Genetic: 99 Phenotypic Enhancement: 1 Phenotypic Suppression: 3 Positive Genetic: 9 Synthetic Growth Defect: 7 Synthetic Lethality: 4 Synthetic Rescue: 12
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All MKS1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXIV:483556 to 485310 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1755	483556..485310	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005020

References cited on this page View Complete Literature Guide for MKS1

1)	Matsuura A and Anraku Y (1993) Characterization of the MKS1 gene, a new negative regulator of the Ras-cyclic AMP pathway in Saccharomyces cerevisiae. Mol Gen Genet 238(1-2):6-16
2)	Edskes HK, et al. (1999) Mks1p is a regulator of nitrogen catabolism upstream of Ure2p in Saccharomyces cerevisiae. Genetics 153(2):585-94
3)	Feller A, et al. (1997) Lys80p of Saccharomyces cerevisiae, previously proposed as a specific repressor of LYS genes, is a pleiotropic regulatory factor identical to Mks1p. Yeast 13(14):1337-46
4)	Sekito T, et al. (2002) RTG-dependent mitochondria-to-nucleus signaling is regulated by MKS1 and is linked to formation of yeast prion [URE3]. Mol Biol Cell 13(3):795-804