MID2/YLR332W Summary Help

Standard Name MID2 1
Systematic Name YLR332W
Alias KAI1
Feature Type ORF, Verified
Description O-glycosylated plasma membrane protein; acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p; MID2 has a paralog, MTL1, that arose from the whole genome duplication (2, 3, 4)
Name Description Mating pheromone-Induced Death 5
Chromosomal Location
ChrXII:790676 to 791806 | ORF Map | GBrowse
Gene Ontology Annotations All MID2 GO evidence and references
  View Computational GO annotations for MID2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 7 genes
Classical genetics
Large-scale survey
211 total interaction(s) for 138 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 3
  • Co-purification: 1
  • PCA: 48
  • Protein-RNA: 2
  • Reconstituted Complex: 2
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 33
  • Negative Genetic: 65
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 5
  • Positive Genetic: 2
  • Synthetic Growth Defect: 11
  • Synthetic Lethality: 25
  • Synthetic Rescue: 3

Expression Summary
Length (a.a.) 376
Molecular Weight (Da) 39,146
Isoelectric Point (pI) 5.37
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:790676 to 791806 | ORF Map | GBrowse
Last Update Coordinates: 2004-02-05 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1131 790676..791806 2004-02-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004324
References cited on this page View Complete Literature Guide for MID2
1) Ono T, et al.  (1994) The MID2 gene encodes a putative integral membrane protein with a Ca(2+)-binding domain and shows mating pheromone-stimulated expression in Saccharomyces cerevisiae. Gene 151(1-2):203-8
2) Philip B and Levin DE  (2001) Wsc1 and Mid2 are cell surface sensors for cell wall integrity signaling that act through Rom2, a guanine nucleotide exchange factor for Rho1. Mol Cell Biol 21(1):271-80
3) Green R, et al.  (2003) A synthetic analysis of the Saccharomyces cerevisiae stress sensor Mid2p, and identification of a Mid2p-interacting protein, Zeo1p, that modulates the PKC1-MPK1 cell integrity pathway. Microbiology 149(Pt 9):2487-99
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Iida H, et al.  (1994) MID1, a novel Saccharomyces cerevisiae gene encoding a plasma membrane protein, is required for Ca2+ influx and mating. Mol Cell Biol 14(12):8259-71